Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry.

Détails

ID Serval
serval:BIB_68081043E7D7
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry.
Périodique
Journal of the American Society For Mass Spectrometry
Auteur⸱e⸱s
Roussel C., Dayon L., Lion N., Rohner T.C., Josserand J., Rossier J.S., Jensen H., Girault H.H.
ISSN
1044-0305 (Print)
ISSN-L
1044-0305
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
15
Numéro
12
Pages
1767-1779
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review Publication Status: ppublish
Résumé
We present herein a review of our work on the on-line electrochemical generation of mass tags toward cysteine residues in peptides and proteins. Taking advantage of the inherent electrochemical nature of electrospray generated from a microfabricated microspray emitter, selective probes for cysteine were developed and tested for on-line nonquantitative mass tagging of peptides and proteins. The nonquantitative aspect of the covalent tagging thus allows direct counting of free cysteines in the mass spectrum of a biomolecule through additional adduct peaks. Several substituted hydroquinones were investigated in terms of electrochemical properties, and their usefulness for on-line mass tagging during microspray experiments were assessed with L-cysteine, peptides, and intact proteins. Complementarily, numerical simulations were performed to properly understand the respective roles of mass transport, kinetics of electrochemical-chemical reactions, and design of the microspray emitter in the mass tagging overall efficiency. Finally, the on-line electrochemical tagging of cysteine residues was applied to the analysis of tryptic peptides of purified model proteins for protein identification through peptide mass fingerprinting.
Mots-clé
Cysteine/analysis, Electrochemistry, Microchemistry/methods, Peptides/chemistry, Proteins/chemistry, Spectrometry, Mass, Electrospray Ionization/instrumentation, Spectrometry, Mass, Electrospray Ionization/methods
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/11/2014 22:49
Dernière modification de la notice
20/08/2019 14:23
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