Membrane activity of a C-reactive protein.
Détails
ID Serval
serval:BIB_68071F7A91B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Membrane activity of a C-reactive protein.
Périodique
FEBS letters
ISSN
1873-3468 (Electronic)
ISSN-L
0014-5793
Statut éditorial
Publié
Date de publication
18/03/2009
Peer-reviewed
Oui
Volume
583
Numéro
6
Pages
1001-1005
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Résumé
C-reactive protein (CRP) from the American horseshoe crab, Limulus polyphemus, exhibits complex membrane activities. Here, we describe the behavior of protein and lipid as CRP interacts with model liposomes and bacterial membranes. Limulus C-reactive protein (L-CRP) forms extended fibrilar structures that encapsulate liposomes in the presence of Ca(2+). We have observed structures consistent in size and shape with these fibers bound to the surface of Gram-negative bacteria. The membranes of Limulus CRP-treated bacteria exhibit significantly different mechano-elastic properties than those of untreated bacteria. In vitro, bilayer lipids undergo a rigidification and reorganization of small domains. We suggest that these interactions reflect the protein's role as a primary defense molecule, functioning in the entrapment and killing of potential pathogens.
Mots-clé
Agglutination/drug effects, Agglutination Tests, Animals, Bacteria/metabolism, C-Reactive Protein/metabolism, Calcium/pharmacology, Cell Membrane/metabolism, Computer Simulation, Horseshoe Crabs, Lipid Bilayers/metabolism, Liposomes/metabolism, Membrane Lipids/metabolism, Protein Binding/drug effects
Pubmed
Web of science
Création de la notice
09/06/2023 15:03
Dernière modification de la notice
28/07/2023 5:59