Hierarchical management of carbon sources is regulated similarly by the CbrA/B systems in Pseudomonas aeruginosa and Pseudomonas putida.

Détails

ID Serval
serval:BIB_6790E1073684
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Hierarchical management of carbon sources is regulated similarly by the CbrA/B systems in Pseudomonas aeruginosa and Pseudomonas putida.
Périodique
Microbiology
Auteur⸱e⸱s
Valentini M., García-Mauriño S.M., Pérez-Martínez I., Santero E., Canosa I., Lapouge K.
ISSN
1465-2080 (Electronic)
ISSN-L
1350-0872
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
160
Numéro
Pt 10
Pages
2243-2252
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The CbrA/B system in pseudomonads is involved in the utilization of carbon sources and carbon catabolite repression (CCR) through the activation of the small RNAs crcZ in Pseudomonas aeruginosa, and crcZ and crcY in Pseudomonas putida. Interestingly, previous works reported that the CbrA/B system activity in P. aeruginosa PAO1 and P. putida KT2442 responded differently to the presence of different carbon sources, thus raising the question of the exact nature of the signal(s) detected by CbrA. Here, we demonstrated that the CbrA/B/CrcZ(Y) signal transduction pathway is similarly activated in the two Pseudomonas species. We show that the CbrA sensor kinase is fully interchangeable between the two species and, moreover, responds similarly to the presence of different carbon sources. In addition, a metabolomics analysis supported the hypothesis that CCR responds to the internal energy status of the cell, as the internal carbon/nitrogen ratio seems to determine CCR and non-CCR conditions. The strong difference found in the 2-oxoglutarate/glutamine ratio between CCR and non-CCR conditions points to the close relationship between carbon and nitrogen availability, or the relationship between the CbrA/B and NtrB/C systems, suggesting that both regulatory systems sense the same sort or interrelated signal.
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/12/2014 10:34
Dernière modification de la notice
20/08/2019 15:23
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