DNA methylation profiling of esophageal adenocarcinoma using Methylation Ligation-dependent Macroarray (MLM).

Détails

ID Serval
serval:BIB_677D1CA0B298
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
DNA methylation profiling of esophageal adenocarcinoma using Methylation Ligation-dependent Macroarray (MLM).
Périodique
Biochemical and Biophysical Research Communications
Auteur⸱e⸱s
Guilleret I., Losi L., Chelbi S.T., Fonda S., Bougel S., Saponaro S., Gozzi G., Alberti L., Braunschweig R., Benhattar J.
ISSN
1090-2104 (Electronic)
ISSN-L
0006-291X
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
479
Numéro
2
Pages
231-237
Langue
anglais
Notes
Publication types: ARTICLEPublication Status: ppublish
Résumé
Most types of cancer cells are characterized by aberrant methylation of promoter genes. In this study, we described a rapid, reproducible, and relatively inexpensive approach allowing the detection of multiple human methylated promoter genes from many tissue samples, without the need of bisulfite conversion. The Methylation Ligation-dependent Macroarray (MLM), an array-based analysis, was designed in order to measure methylation levels of 58 genes previously described as putative biomarkers of cancer. The performance of the design was proven by screening the methylation profile of DNA from esophageal cell lines, as well as microdissected formalin-fixed and paraffin-embedded (FFPE) tissues from esophageal adenocarcinoma (EAC). Using the MLM approach, we identified 32 (55%) hypermethylated promoters in EAC, and not or rarely methylated in normal tissues. Among them, 21promoters were found aberrantly methylated in more than half of tumors. Moreover, seven of them (ADAMTS18, APC, DKK2, FOXL2, GPX3, TIMP3 and WIF1) were found aberrantly methylated in all or almost all the tumor samples, suggesting an important role for these genes in EAC. In addition, dysregulation of the Wnt pathway with hypermethylation of several Wnt antagonist genes was frequently observed. MLM revealed a homogeneous pattern of methylation for a majority of tumors which were associated with an advanced stage at presentation and a poor prognosis. Interestingly, the few tumors presenting less methylation changes had a lower pathological stage. In conclusion, this study demonstrated the feasibility and accuracy of MLM for DNA methylation profiling of FFPE tissue samples.
Pubmed
Web of science
Création de la notice
20/09/2016 7:35
Dernière modification de la notice
20/08/2019 14:23
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