Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1.

Détails

Ressource 1Télécharger: 2021_Gerganova_MBoC.pdf (1957.98 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_677BA0EC374C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1.
Périodique
Molecular biology of the cell
Auteur(s)
Gerganova V., Bhatia P., Vincenzetti V., Martin S.G.
ISSN
1939-4586 (Electronic)
ISSN-L
1059-1524
Statut éditorial
Publié
Date de publication
15/04/2021
Peer-reviewed
Oui
Volume
32
Numéro
8
Pages
703-711
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The fission yeast cells Schizosaccharomyces pombe divide at constant cell size regulated by environmental stimuli. An important pathway of cell size control involves the membrane-associated DYRK-family kinase Pom1, which forms decreasing concentration gradients from cell poles and inhibits mitotic inducers at midcell. Here, we identify the phosphatase 2C Ptc1 as negative regulator of Pom1. Ptc1 localizes to cell poles in a manner dependent on polarity and cell-wall integrity factors. We show that Ptc1 directly binds Pom1 and can dephosphorylate it in vitro but modulates Pom1 localization indirectly upon growth in low-glucose conditions by influencing microtubule stability. Thus, Ptc1 phosphatase plays both direct and indirect roles in the Pom1 cell size control pathway.
Pubmed
Web of science
Création de la notice
01/03/2021 11:14
Dernière modification de la notice
07/07/2021 6:10
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