Can GAP-43 interact with brain spectrin?

Détails

ID Serval
serval:BIB_675EFC11023E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Can GAP-43 interact with brain spectrin?
Périodique
Brain Research. Molecular Brain Research
Auteur(s)
Riederer B.M., Routtenberg A.
ISSN
0169-328X (Print)
ISSN-L
0169-328X
Statut éditorial
Publié
Date de publication
1999
Volume
71
Numéro
2
Pages
345-348
Langue
anglais
Résumé
The growth-associated and presynaptic protein GAP-43 is important for axonal growth during brain development, for synaptic plasticity and in axonal regeneration [Benowitz, Routtenberg, TINS 12 (1987) 527]. It has been speculated that such growth may be mediated by cytoskeletal proteins. However, the interaction of GAP-43 with proteins of the presynaptic terminals is poorly characterized. Here, we analyze GAP-43 binding to cytoskeletal proteins by two different biochemical assays, by blot overlay and sedimentation. We find that immobilized brain spectrin (BS) is able to bind GAP-43. In contrast, little binding was observed to microtubule proteins and other elements of the cytoskeleton. Since GAP-43 is located presynaptically, it may bind to the presynaptic form of BS (SpIISigma1). It is attractive to think that such an interaction would participate in the structural plasticity observed in growth cones and adult synapses.
Mots-clé
Animals, Biotinylation, Brain/growth & development, Brain/metabolism, Electrophoresis, Polyacrylamide Gel, GAP-43 Protein/metabolism, Rats, Rats, Sprague-Dawley, Spectrin/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 15:34
Dernière modification de la notice
20/08/2019 15:22
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