Immediate shear stress resistance of endothelial cell monolayers seeded in vitro on fibrin glue-coated ePTFE prostheses

Détails

ID Serval
serval:BIB_668D17795E34
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Immediate shear stress resistance of endothelial cell monolayers seeded in vitro on fibrin glue-coated ePTFE prostheses
Périodique
European Journal of Vascular Surgery
Auteur(s)
Muller-Glauser  W., Zilla  P., Lachat  M., Bisang  B., Rieser  F., von Segesser  L., Turina  M.
ISSN
1078-5884
0950-821X
Statut éditorial
Publié
Date de publication
05/1993
Volume
7
Numéro
3
Pages
324-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
The shear stress resistance of endothelial cells (EC) previously seeded onto ePTFE grafts was assessed by morphometric determination of the number of cells per cm2 of graft surface before and after exposure of 6 h of arterial blood flow interposed in the canine femoral artery. Autologous venous endothelial cells (AVEC) were harvested from the extrajugular veins of five dogs. The AVEC were cultured in vitro and seeded at a density of 150 x 10(3) cells per cm2 onto 4 mm ID ePTFE grafts precoated with fibrin glue and human fibronectin. Subsequently, the AVEC monolayers on the grafts were cultured for 8 days using a perfusion system and then implanted end-to-end in the femoral artery. All grafts remained patent (5/5). Scanning electron microscopy demonstrated complete, thrombus-free monolayers of AVEC after 6 h of arterial blood flow. The cell densities were 124 +/- 14 and 129 +/- 7 x 10(3) cells per cm2 respectively before and after implantation. It is concluded that in vitro lining of 4 mm ePTFE vascular prostheses is feasible and results in EC monolayers on the graft surface which are shear stress resistant and athrombogenic.
Mots-clé
Animals Blood Flow Velocity/physiology *Blood Vessel Prosthesis Dogs Endothelium, Vascular/pathology/*physiopathology *Fibrin Tissue Adhesive Graft Occlusion, Vascular/pathology/physiopathology Microscopy, Electron, Scanning Models, Cardiovascular *Polytetrafluoroethylene Prosthesis Design Surface Properties Vascular Resistance/*physiology
Pubmed
Web of science
Création de la notice
14/02/2008 14:17
Dernière modification de la notice
20/08/2019 14:22
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