An orthotopic floor-of-mouth cancer model allows quantification of tumor invasion.

Détails

ID Serval
serval:BIB_667CDC00654B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An orthotopic floor-of-mouth cancer model allows quantification of tumor invasion.
Périodique
Laryngoscope
Auteur⸱e⸱s
Simon C., Nemechek A.J., Boyd D., O'Malley B.W., Goepfert H., Flaitz C.M., Hicks M.J.
ISSN
0023-852X (Print)
ISSN-L
0023-852X
Statut éditorial
Publié
Date de publication
1998
Volume
108
Numéro
11 Pt 1
Pages
1686-1691
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
OBJECTIVES: To establish an orthotopic murine floor-of-mouth cancer model for the analysis of the role of proteases such as urokinase-type plasminogen activator (u-PA) and the matrix metalloprotease MMP-9 (MMP-9) in in vivo invasion.
STUDY DESIGN: Randomized, prospective animal study.
METHODS: Two human squamous cell carcinoma cell lines, UM-SCC-1 and 022, were assayed via zymography for their in vitro secretion levels of u-PA and MMP-9. Both cell lines (5 x 10(6) cells) were injected into the cervical subcutaneous tissues of female athymic nude (nu/nu) mice superficial to the mylohyoid muscle. Mice were sacrificed after 30 days, and tumor invasion characteristics were histologically compared. Additional mice were then inoculated with invasive UM-SCC-1 cells and sacrificed 10, 30, and 40 days after inoculation to identify distinct stages of invasion.
RESULTS: In vitro secretion levels of MMP-9 and activity of u-PA were higher in UM-SCC-1 cells than in 022 cells. In the in vivo studies, tumors formed from 022 cells were found to be noninvasive, whereas tumors derived from UM-SCC-1 cells progressed through distinct and readily identifiable histologic stages of invasion. These stages included invasion of adjacent muscle layers (mylohyoid, geniohyoid, and genioglossus muscles) and of associated structures (blood vessels, bone, nerve, and regional lymph nodes). A staging system was devised accordingly.
CONCLUSION: We developed an in vivo quantitative cancer invasion model that allows determination of the effect of the expression and activity levels of the proteases MMP-9 and u-PA. Tumor invasion occurred in an orderly and stepwise fashion involving muscles and related vascular, nervous, and bony structures of the floor of the mouth and tongue. This orderly invasion allowed the development of a staging system. We anticipate that this model will have wide applicability in the study of in vivo tumor response to a variety of novel therapeutic approaches.
Mots-clé
Animals, Carcinoma, Squamous Cell/enzymology, Carcinoma, Squamous Cell/pathology, Collagenases/analysis, Collagenases/genetics, Disease Models, Animal, Female, Follow-Up Studies, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Humans, Injections, Intramuscular, Lymph Nodes/pathology, Mandible/pathology, Matrix Metalloproteinase 9, Mice, Mice, Nude, Mouth Floor/pathology, Mouth Neoplasms/enzymology, Mouth Neoplasms/pathology, Neck Muscles/blood supply, Neck Muscles/innervation, Neoplasm Invasiveness, Neoplasm Staging, Neoplasm Transplantation, Plasminogen Activators/analysis, Plasminogen Activators/genetics, Prospective Studies, Random Allocation, Tongue/pathology, Tumor Cells, Cultured, Urokinase-Type Plasminogen Activator/analysis, Urokinase-Type Plasminogen Activator/genetics
Pubmed
Web of science
Création de la notice
21/01/2013 13:53
Dernière modification de la notice
20/08/2019 14:22
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