Influence of experimental parameters on in vitro human skin permeation of Bisphenol A.
Détails
Télécharger: 2021_Reale_Influence_ToxicologyVitro_105129.pdf (772.32 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_65AA780564EB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Influence of experimental parameters on in vitro human skin permeation of Bisphenol A.
Périodique
Toxicology in vitro
ISSN
1879-3177 (Electronic)
ISSN-L
0887-2333
Statut éditorial
Publié
Date de publication
06/2021
Peer-reviewed
Oui
Volume
73
Pages
105129
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Bisphenol A (BPA) in vitro skin permeation studies have shown inconsistent results, which could be due to experimental conditions. We studied the impact of in vitro parameters on BPA skin permeation using flow-through diffusion cells with ex-vivo human skin (12 donors, 3-12 replicates). We varied skin status (viable or frozen skin) and thickness (200, 400, 800 μm), BPA concentrations (18, 250 mg/l) and vehicle volumes (10, 100 and 1000 μl/cm <sup>2</sup> ). These conditions led to a wide range of BPA absorption (2%-24% after 24 h exposure), peak permeation rates (J = 0.02-1.31 μg/cm <sup>2</sup> /h), and permeability coefficients (K <sub>p</sub> = 1.6-5.2 × 10 <sup>-3</sup> cm/h). This is the first time steady state conditions were reached for BPA aqueous solutions in vitro (1000 μl/cm <sup>2</sup> applied at concentration 250 mg/l). A reduction of the skin thickness from 800 and 400 μm to 200 μm led to a 3-fold increase of J (P < 0.05). A reduction of the vehicle volume from 1000 to 100 led to a 2-fold decrease in J (P > 0.05). Previously frozen skin led to a 3-fold increase in J compared to viable skin (P < 0.001). We found that results from published studies were consistent when adjusting J according to experimental parameters. We propose appropriate J values for different exposure scenarios to calculate BPA internal exposures for use in risk assessment.
Mots-clé
Administration, Cutaneous, Benzhydryl Compounds/pharmacology, Humans, In Vitro Techniques, Phenols/pharmacology, Skin/anatomy & histology, Skin/metabolism, Skin Absorption, BPA, Human skin, Percutaneous absorption, Permeation constant, Skin thickness, Vehicle volume
Pubmed
Web of science
Site de l'éditeur
Création de la notice
04/03/2021 18:12
Dernière modification de la notice
21/04/2022 7:10