Immunoscintigraphic localization of inflammatory lesions: concept, radiolabelling and in vitro testing of a granulocyte specific antibody.

Détails

ID Serval
serval:BIB_6547CDEF5B31
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Immunoscintigraphic localization of inflammatory lesions: concept, radiolabelling and in vitro testing of a granulocyte specific antibody.
Périodique
European Journal of Nuclear Medicine
Auteur⸱e⸱s
Andres R.Y., Schubiger P.A., Tiefenauer L., Seybold K., Locher J.T., Mach J.P., Buchegger F.
ISSN
0340-6997 (Print)
ISSN-L
0340-6997
Statut éditorial
Publié
Date de publication
1988
Peer-reviewed
Oui
Volume
13
Numéro
11
Pages
582-586
Langue
anglais
Résumé
Current nuclear medicine techniques for the localization of inflammatory processes are based on injection of 111In labelled autologous granulocytes which need to be isolated and radiolabelled in vitro before reinjection. A new technique is presented here that obviates the need for cell isolation by the direct intravenous injection of a granulocyte specific 123I labelled monoclonal antibody. In this publication the basic parameters of the antibody granulocyte interaction are described. Antibody binding does not inhibit vital functions of the granulocytes, such as chemotaxis and superoxide generation. Scatchard analysis of binding data reveals an apparent affinity of the antibody for granulocytes of 6.8 X 10(9) l/mol and approximately 7.1 X 10(4) binding sites per cell. Due to the high specificity of the antibody, the only expected interference is from CEA producing tumors.
Mots-clé
Animals, Antibodies, Monoclonal/diagnostic use, Antibodies, Monoclonal/metabolism, Antibody Affinity, Carcinoembryonic Antigen/immunology, Chemotaxis, Leukocyte, Female, Granulocytes/immunology, Granulocytes/metabolism, Humans, Inflammation/radionuclide imaging, Iodine Radioisotopes/diagnostic use, Methods, Species Specificity
Pubmed
Web of science
Création de la notice
25/01/2008 12:27
Dernière modification de la notice
20/08/2019 15:21
Données d'usage