Bacillus subtilis cell cycle as studied by fluorescence microscopy: constancy of cell length at initiation of DNA replication and evidence for active nucleoid partitioning

Détails

ID Serval
serval:BIB_6516A0E8A6BB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Bacillus subtilis cell cycle as studied by fluorescence microscopy: constancy of cell length at initiation of DNA replication and evidence for active nucleoid partitioning
Périodique
Journal of Bacteriology
Auteur⸱e⸱s
Sharpe  M. E., Hauser  P. M., Sharpe  R. G., Errington  J.
ISSN
0021-9193 (Print)
Statut éditorial
Publié
Date de publication
02/1998
Volume
180
Numéro
3
Pages
547-55
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
Fluorescence microscopic methods have been used to characterize the cell cycle of Bacillus subtilis at four different growth rates. The data obtained have been used to derive models for cell cycle progression. Like that of Escherichia coli, the period required by B. subtilis for chromosome replication at 37 degrees C was found to be fairly constant (although a little longer, at about 55 min), as was the cell mass at initiation of DNA replication. The cell cycle of B. subtilis differed from that of E. coli in that changes in growth rate affected the average cell length but not the width and also in the relative variability of period between termination of DNA replication and septation. Overall movement of the nucleoid was found to occur smoothly, as in E. coli, but other aspects of nucleoid behavior were consistent with an underlying active partitioning machinery. The models for cell cycle progression in B. subtilis should facilitate the interpretation of data obtained from the recently introduced cytological methods for imaging the assembly and movement of proteins involved in cell cycle dynamics.
Mots-clé
Bacillus subtilis/*cytology/*genetics/growth & development/metabolism Cell Cycle Chromosomes, Bacterial/*metabolism Culture Media *DNA Replication DNA, Bacterial/analysis/biosynthesis Microscopy, Fluorescence Models, Biological
Pubmed
Web of science
Création de la notice
25/01/2008 14:30
Dernière modification de la notice
20/08/2019 14:21
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