Lead acetate toxicity in vitro: Dependence on the cell composition of the cultures.

Détails

ID Serval
serval:BIB_644CF6AA2437
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Lead acetate toxicity in vitro: Dependence on the cell composition of the cultures.
Périodique
Toxicology in Vitro
Auteur⸱e⸱s
Zurich M.G., Monnet-Tschudi F., Bérode M., Honegger P.
ISSN
0887-2333 (Print)
ISSN-L
0887-2333
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
12
Numéro
2
Pages
191-196
Langue
anglais
Résumé
It is well known that exposure to low doses of lead causes long-lasting neurobehavioural deficits, but the cellular changes underlying these behavioural changes remain to be elucidated. A protective role of glial cells on neurons through lead sequestration by astrocytes has been proposed. The possible modulation of lead neurotoxicity by neuron-glia interactions was examined in three-dimensional cultures of foetal rat telencephalon. Mixed-brain cell cultures or cultures enriched in either neurons or glial cells were treated for 10 days with lead acetate (10(-6) m), a concentration below the limit of cytotoxicity. Intracellular lead content and cell type-specific enzyme activities were determined. It was found that in enriched cultures neurons stored more lead than glial cells, and each cell type alone stored more lead than in co-culture. Moreover, glial cells but not neurons were more affected by lead in enriched culture than in co-culture. These results show that neuron-glia interactions attenuate the cellular lead uptake and the glial susceptibility to lead, but they do not support the idea of a protective role of astrocytes.
Pubmed
Web of science
Création de la notice
24/01/2008 14:12
Dernière modification de la notice
20/08/2019 15:20
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