Germ cell tumors of the testis overexpress wild-type p53

Détails

ID Serval
serval:BIB_641F54AB0D86
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Germ cell tumors of the testis overexpress wild-type p53
Périodique
American Journal of Pathology
Auteur(s)
Guillou  L., Estreicher  A., Chaubert  P., Hurlimann  J., Kurt  A. M., Metthez  G., Iggo  R., Gray  A. C., Jichlinski  P., Leisinger  H. J., Benhattar  J.
ISSN
0002-9440
Statut éditorial
Publié
Date de publication
10/1996
Peer-reviewed
Oui
Volume
149
Numéro
4
Pages
1221-1228
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Several recent studies have suggested that testicular germ cell tumors express high levels of wild-type p53 protein. To clarify and confirm this unexpected result, we have investigated seminomatous and nonseminomatous germ cell tumors at the genomic, mRNA, and protein levels. Thirty-five tumors were examined for p53 overexpression using antibodies directed against the p53 (PAb1801, PAb240, and CM1), mdm2 (IF2), and p21Waf1/Clp1 (EA10) proteins. Thirty-two tumors were screened for p53 mutations by single-strand conformation polymorphism analysis. Eighteen tumors were screened with a functional assay that tests the transcriptional competence of human p53 protein expressed in yeast. On frozen sections, 100, 65, 35, 73, and 0% of tumors reacted with the CM1, PAb240, PAb1801, IF2, and EA10 antibodies, respectively. No p53 mutations were detected by single-strand conformation polymorphism or by functional assay. The fact that many tumors overexpress wild-type p53 but not mdm2 rules out mdm2 overexpression as a general explanation for the presence of wild-type p53 in these tumors. The absence of p21 overexpression suggests that p53 may be unable to activate transcription of critical target genes, which may explain why the presence of wild-type p53 is tolerated in this tumor type, although the mechanism for this transcriptional inactivity remains to be established.
Mots-clé
Cyclin-Dependent Kinase Inhibitor p21 Cyclins/metabolism DNA Mutational Analysis DNA, Neoplasm/genetics Germinoma/genetics/*metabolism/pathology Humans Male Testicular Neoplasms/genetics/*metabolism/pathology Transfection Tumor Suppressor Protein p53/genetics/*metabolism Yeasts/genetics
Pubmed
Web of science
Création de la notice
21/01/2008 17:10
Dernière modification de la notice
20/08/2019 15:20
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