Membrane attack complex (MAC) deposits in skin are not always accompanied by S-protein and clusterin
Détails
ID Serval
serval:BIB_63C0642EFC48
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Membrane attack complex (MAC) deposits in skin are not always accompanied by S-protein and clusterin
Périodique
Journal of Investigative Dermatology
ISSN
0022-202X (Print)
Statut éditorial
Publié
Date de publication
05/1992
Volume
98
Numéro
5
Pages
758-63
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Clusterin and S-protein bind to the membrane attack complex of complement (MAC) rendering it cytolytically inactive. Tissue necrosis as produced by pulsed tunable dye laser therapy (PTDL), and immune complex-related diseases such as lupus erythematosus, are accompanied by local accumulation of MAC. However, the mechanisms responsible for this accumulation might differ, and lead to deposition of MAC in different forms (cytolytically active or inactive). Biopsy specimens of lesional (22) and non-lesional (10) skin from 27 patients with a positive lupus band test (LBT) were studied using monoclonal antibodies against clusterin, S-protein, and MAC by immunofluorescence and immunoperoxidase. Identical studies were performed in normal and angiomatous skin specimens from three normal individuals before and after laser irradiation. MAC was present in 30 of 32 positive LBT skin biopsies. MAC was not only present in lesional (21 of 22) but also in non-lesional skin (nine of 10), although the intensity of staining appeared to be lower in the latter. Clusterin and S-protein co-localized with MAC, respectively, in 20 and 12 specimens, and were not found in the absence of MAC. In addition S-protein deposits were seen only in biopsies positive for clusterin. Deposits of clusterin and S-protein did not correlate with the presence or absence of lesions. After irradiation with PTDL, the immediate complement activation was accompanied by MAC deposits that were granular and clearly located on vascular endothelial cells. Clusterin and S-protein were not present on these cells. In summary, clusterin localizes with MAC along the skin dermal-epidermal junction in patients with a positive LBT, suggesting that it has a similar and possibly more important role than S-protein in regulating immune complex-mediated MAC formation. By contrast, clusterin and S-protein are not involved at the time of MAC formation in cells undergoing necrosis after PTDL therapy.
Mots-clé
Biopsy
Chronic Disease
Clusterin
Complement C3/analysis
Complement Membrane Attack Complex/*analysis
Fluorescent Antibody Technique
Glycoproteins/analysis
Humans
Immunoenzyme Techniques
Lasers/therapeutic use
Lupus Erythematosus, Cutaneous/pathology
Lupus Erythematosus, Systemic/pathology
*Molecular Chaperones
Skin/*chemistry/pathology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:18
Dernière modification de la notice
20/08/2019 14:20