Cyclic AMP induces differentiation in vitro of human melanoma cells.

Détails

ID Serval
serval:BIB_6357C112FE69
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cyclic AMP induces differentiation in vitro of human melanoma cells.
Périodique
Cancer
Auteur(s)
Giuffrè L., Schreyer M., Mach J.P., Carrel S.
ISSN
0008-543X
Statut éditorial
Publié
Date de publication
1988
Peer-reviewed
Oui
Volume
61
Numéro
6
Pages
1132-1141
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Treating human melanoma lines with dibutyryl adenosine 3':5'-cyclic monophosphate (dbc AMP) resulted in morphologic changes associated with the altered expression of cell surface antigens. After treatment, cells developed long cellular projections characteristic of mature melanocytes and showed the presence of an increased number of Stage II premelanosomes. In addition, induction of melanin synthesis, detected as brown perinuclear pigmentation, was observed. The AMP further drastically reduced the growth rate of the five melanoma cell lines that were tested. The influence of dbc AMP was completely reversible 3 days after the agent was removed from the culture medium. The antigenic phenotype of the melanoma lines was compared before and after dbc AMP treatment. This was done with four monoclonal antibodies directed against major histocompatibility complex (MHC) Class I and II antigens and 11 monoclonal antibodies defining eight different melanoma-associated antigenic systems. Treatment with dbc AMP reduced the expression of human leukocyte antigen (HLA)-ABC antigens and beta-2-microglobulin in five of five melanoma lines. In the two HLA-DR-positive cell lines dbc AMP reduced the expression of this antigen in one line and enhanced it in the other. No induction of HLA-DR or HLA-DC antigens was observed in the Class II negative cell lines. Furthermore, dbc-AMP modulated the expression of the majority of the melanoma antigenic systems tested. The expression of a 90-kilodalton (KD) antigen, which has been found to be upregulated by interferon-gamma, was markedly decreased in all the five cell lines. A similar decrease in the expression of the high molecular weight proteoglycan-associated antigen (220-240 KD) was observed. The reduced expression of Class I and II MHC antigens as well as the altered expression of the melanoma-associated antigens studied were shown to be reversible after dbc AMP was removed. Our results collectively show that the monoclonal antibody-defined melanoma-associated molecules are linked to differentiation. They could provide useful tools for monitoring the maturation of melanomas in vivo induced by chemical agents or natural components favoring differentiation.
Mots-clé
Antigens, Neoplasm/biosynthesis, Antigens, Surface/biosynthesis, Bucladesine/pharmacology, Cell Differentiation/drug effects, Cell Line, Humans, Melanins/biosynthesis, Melanoma/pathology, Tumor Cells, Cultured/drug effects
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2009 12:36
Dernière modification de la notice
20/08/2019 15:19
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