Employing an orthotopic model to study the role of epithelial-mesenchymal transition in bladder cancer metastasis.

Détails

ID Serval
serval:BIB_62307F91C3C7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Employing an orthotopic model to study the role of epithelial-mesenchymal transition in bladder cancer metastasis.
Périodique
Oncotarget
Auteur(s)
Roth B., Jayaratna I., Sundi D., Cheng T., Melquist J., Choi W., Porten S., Nitti G., Navai N., Wszolek M., Guo C., Czerniak B., McConkey D., Dinney C.
ISSN
1949-2553 (Electronic)
ISSN-L
1949-2553
Statut éditorial
Publié
Date de publication
23/05/2017
Peer-reviewed
Oui
Volume
8
Numéro
21
Pages
34205-34222
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Epithelial-to-mesenchymal transition (EMT) has been implicated in the progression of bladder cancer. To study its contribution to bladder cancer metastasis, we established new xenograft models derived from human bladder cancer cell lines utilizing an orthotopic "recycling" technique that allowed us to isolate and examine the primary tumor and its corresponding circulating tumor cells (CTC's) and metastatic lesions. Using whole genome mRNA expression profiling, we found that a reversible epithelial-to-mesenchymal transition (EMT) characterized by TGFβ pathway activation and SNAIL expression was associated with the accumulation of CTCs. Finally, we observed that conditional silencing of SNAIL completely blocked CTC production and regional/distant metastasis. Using this unique bladder cancer xenograft model, we conclude that metastasis is dependent on a reversible EMT mediated by SNAIL.
Mots-clé
Animals, Cell Line, Tumor, Disease Models, Animal, Disease Progression, Epithelial-Mesenchymal Transition, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Neoplasm Metastasis, Neoplastic Cells, Circulating/metabolism, Neoplastic Cells, Circulating/pathology, Signal Transduction/drug effects, Snail Family Transcription Factors/genetics, Snail Family Transcription Factors/metabolism, Transforming Growth Factor beta/genetics, Transforming Growth Factor beta/metabolism, Urinary Bladder Neoplasms/genetics, Urinary Bladder Neoplasms/metabolism, Urinary Bladder Neoplasms/pathology, Whole Genome Sequencing, SNAIL, bladder cancer, circulating tumor cells, metastasis, orthotopic xenografts
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/01/2021 20:10
Dernière modification de la notice
09/01/2021 7:26
Données d'usage