Induction of transendothelial migration in normal and malignant human T lymphocytes.

Détails

ID Serval
serval:BIB_62201DF732A9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Induction of transendothelial migration in normal and malignant human T lymphocytes.
Périodique
Anticancer Research
Auteur(s)
Hauzenberger D., Hultenby K., Sumitran S., Ruegg C., Klominek J.
ISSN
0250-7005 (Print)
ISSN-L
0250-7005
Statut éditorial
Publié
Date de publication
2000
Volume
20
Numéro
4
Pages
2601-2611
Langue
anglais
Résumé
Activated CD 3+ enriched human peripheral blood T cells exhibited potent capacity for transendothelial migration through HUVEC layers in the absence of T cell ***. In contrast, malignant human T cell lines *** no or negligible ability of transendothelial migration in the absence of chemoattractants. Time lapse studies of transendothelial migration of activated CD 3+ enriched peripheral blood T cells through a HUVEC layer showed that the first T cells were detected in the lower compartment of a tissue culture insert after 1 hour and that migration increased to reach a maximum of 25 x 10(4) T cells/hr after 24 hours. Adhesion assays of human T cell lines demonstrated that all T cell lines were capable of adhesion to HUVEC and that adhesion of T cells to HUVECs was primarily mediated by CD11a/CD18 and ICAM-1 interactions. Furthermore, transendothelial migration of CD 3+ enriched human peripheral blood T cells was inhibited by pretreating the T cells with anti-CD 18 monoclonal antibodies. The inability of malignant T cells to migrate through HUVEC layers in the absence of chemoattractants was not due to poor motility per se, since both normal and malignant T cells migrated well on extracellular matrix components as determined by using Boyden chambers. Crosslinking of alpha 1 beta 2 and alpha 4 beta 1 with immobilized monoclonal antibodies induced motile behaviour in activated CD 3 enriched human peripheral blood T cells but not in malignant T cell lines. In conclusion, the differences in the ability of transendothelial migration between normal and malignant human T cells in the absence of chemoattractants is primarily due to the differences in the capacity of alpha 1 beta 2 and alpha 4 beta 1 to trigger motile behaviour in the separate cell types.
Mots-clé
Adult, Cell Adhesion, Cell Movement, Cells, Cultured, Endothelium, Vascular/cytology, Extracellular Matrix Proteins/physiology, Humans, Integrins/analysis, T-Lymphocytes/physiology, Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
28/01/2008 8:36
Dernière modification de la notice
20/08/2019 14:19
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