Revised locations of the hisI and pru (proline utilization) genes on the Pseudomonas aeruginosa chromosome map.
Détails
ID Serval
serval:BIB_61E48EFDF589
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Revised locations of the hisI and pru (proline utilization) genes on the Pseudomonas aeruginosa chromosome map.
Périodique
Molecular and General Genetics
ISSN
0026-8925 (Print)
ISSN-L
0026-8925
Statut éditorial
Publié
Date de publication
1984
Volume
193
Numéro
3
Pages
431-436
Langue
anglais
Résumé
The location of genes in the vicinity of the major FP2 origin on the chromosome of Pseudomonas aeruginosa PAO has been revised. The markers hisI (a transduction group of histidine biosynthetic genes) and pru (a gene cluster encoding proline utilization functions) were located in the 90 to 95/0 min chromosome region by a series of plate matings mediated by R68.45. Three-factor-crosses using this plasmid established the following marker order: pur-67 pru hisI/cys-59 proB ilvB/C. Genetic evidence is presented to confirm the previous observations that FP2 can mobilize the chromosome from at least two origins near proB and in both directions. Thus, when markers in this chromosome region are analyzed by FP2 crosses only, the mapping data may be difficult to interpret. This complication can be overcome by the use of R68.45 and Tfr (transposon-facilitated recombination) or Hfr donors.
Mots-clé
Chromosome Mapping, Chromosomes, Bacterial/physiology, Crosses, Genetic, Genes, Bacterial, Genetic Linkage, Genotype, Histidine/genetics, Plasmids, Proline/genetics, Pseudomonas aeruginosa/genetics, Transduction, Genetic
Pubmed
Web of science
Création de la notice
25/01/2008 17:00
Dernière modification de la notice
20/08/2019 14:18