Poly-N-Acetyl Glucosamine Fibers Induce Angiogenesis in ADP Inhibitor-Treated Diabetic Mice

Détails

ID Serval
serval:BIB_616D874EC4B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Poly-N-Acetyl Glucosamine Fibers Induce Angiogenesis in ADP Inhibitor-Treated Diabetic Mice
Périodique
Journal of Trauma-Injury Infection and Critical Care
Auteur(s)
Scherer S. S., Pietramaggiori G., Matthews J. C., Gennaoui A., Demcheva M., Fischer T. H., Valeri C. R., Orgill D. P.
ISSN
0022-5282
Statut éditorial
Publié
Date de publication
2011
Volume
71
Numéro
2 Suppl 1
Pages
S183-6
Langue
anglais
Résumé
Background: It has been previously demonstrated that short-fiber poly-N-acetyl-glucosamine (sNAG) nanofibers specifically interact with platelets, are hemostatic, and stimulate diabetic wound healing by activating angiogenesis, cell proliferation, and reepithelialization. Platelets play a significant physiologic role in wound healing. The influence of altered platelet function by treatment with the ADP inhibitor Clopidogrel (CL) on wound healing and the ability of sNAG to repair wounds in diabetic mice treated with CL were studied.Methods: Dorsal 1 cm2 skin wounds were excised on genetically diabetic 8-week to 12-week-old, Lep/r-db/db male mice, and wound healing kinetics were determined. Microscopic analysis was performed for angiogenesis (PECAM-1) and cell proliferation (Ki67). Mice were either treated with CL (P2Y12 ADP receptor antagonist, CL) or saline solution (NT). CL wounds were also treated with either a single application of topical sNAG (CL-sNAG) or were left untreated (CL-NT).Results: CL treatment did not alter wound healing kinetics, while sNAG induced faster wound closure in CL-treated mice compared with controls. CL treatment of diabetic mice caused an augmentation of cell proliferation and reduced angiogenesis compared with nontreated wounds. However, sNAG reversed the effects of CL on angiogenesis and partially reversed the effect on cell proliferation in the wound beds. The sNAG-treated wounds in CL-treated mice showed higher levels of cell proliferation and not did inhibit angiogenesis.Conclusions: CL treatment of diabetic mice decreased angiogenesis and increased cell proliferation in wounds but did not influence macroscopic wound healing kinetics. sNAG treatment did not inhibit angiogenesis in CL-treated mice and induced faster wound closure; sNAG technology is a promising strategy to facilitate the healing of complex bleeding wounds in CL-treated diabetic patients.
Mots-clé
Angiogenesis, Diabetic wounds, Poly-N-acetyl glucosamine, Clopidogrel, PLATELET-RICH PLASMA, FOOT ULCERS, CLOPIDOGREL, ACTIVATION
Web of science
Création de la notice
05/09/2011 9:10
Dernière modification de la notice
20/08/2019 15:18
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