Extracellular matrix formation after transplantation of human embryonic stem cell-derived cardiomyocytes.

Détails

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Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_612862AD37D1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Extracellular matrix formation after transplantation of human embryonic stem cell-derived cardiomyocytes.
Périodique
Cellular and Molecular Life Sciences
Auteur(s)
van Laake L.W., van Donselaar E.G., Monshouwer-Kloots J., Schreurs C., Passier R., Humbel B.M., Doevendans P.A., Sonnenberg A., Verkleij A.J., Mummery C.L.
ISSN
1420-9071 (Electronic)
ISSN-L
1420-682X
Statut éditorial
Publié
Date de publication
2010
Volume
67
Numéro
2
Pages
277-290
Langue
anglais
Résumé
Transplantation of human embryonic stem cell-derived cardiomyocytes (hESC-CM) for cardiac regeneration is hampered by the formation of fibrotic tissue around the grafts, preventing electrophysiological coupling. Investigating this process, we found that: (1) beating hESC-CM in vitro are embedded in collagens, laminin and fibronectin, which they bind via appropriate integrins; (2) after transplantation into the mouse heart, hESC-CM continue to secrete collagen IV, XVIII and fibronectin; (3) integrin expression on hESC-CM largely matches the matrix type they encounter or secrete in vivo; (4) co-transplantation of hESC-derived endothelial cells and/or cardiac progenitors with hESC-CM results in the formation of functional capillaries; and (5) transplanted hESC-CM survive and mature in vivo for at least 24 weeks. These results form the basis of future developments aiming to reduce the adverse fibrotic reaction that currently complicates cell-based therapies for cardiac disease, and to provide an additional clue towards successful engraftment of cardiomyocytes by co-transplanting endothelial cells.
Mots-clé
Animals, Cell Differentiation, Cell Line, Embryonic Stem Cells/physiology, Extracellular Matrix/metabolism, Humans, Mice, Myocytes, Cardiac/cytology, Myocytes, Cardiac/transplantation, Neovascularization, Physiologic
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/02/2012 19:58
Dernière modification de la notice
20/08/2019 15:18
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