Human three-dimensional engineered neural tissue reveals cellular and molecular events following cytomegalovirus infection.

Détails

ID Serval
serval:BIB_60CC1E89A633
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Human three-dimensional engineered neural tissue reveals cellular and molecular events following cytomegalovirus infection.
Périodique
Biomaterials
Auteur(s)
Cosset É., Martinez Y., Preynat-Seauve O., Lobrinus J.A., Tapparel C., Cordey S., Peterson H., Petty T.J., Colaianna M., Tieng V., Tirefort D., Dinnyes A., Dubois-Dauphin M., Kaiser L., Krause K.H.
ISSN
1878-5905 (Electronic)
ISSN-L
0142-9612
Statut éditorial
Publié
Date de publication
2015
Volume
53
Pages
296-308
Langue
anglais
Résumé
Human cytomegalovirus (HCMV) is the most common cause of congenital infection of the central nervous system (CNS). To overcome the limited access to human neural tissue and stringent species specificity of HCMV, we used engineered neural tissues to: (i) provide a technical advance to mimick features of HCMV infection in a human neural fetal tissue in vitro and (ii) characterize the molecular and cellular phenomenon following HCMV infection in this tissue. Herein, we infected hESC-derived engineered neural tissues (ENTs) whose organization resembles fetal brain. Transcriptome analysis of ENTs demonstrated that HCMV infection displayed features of the infection with the expression of genes involved in lipid metabolism, growth and development, as well as stress and host-response in a time-dependent manner. Immunohistochemical analysis demonstrated that HCMV did not firstly infect neural tubes (i.e. radially organized, proliferating stem cell niches), but rather an adjacent side population of post-mitotic cells expressing nestin, doublecortin, Sox1, musashi and vimentin markers. Importantly, we observe the same tropism in naturally HCMV-infected fetal brain specimens. To the best of our knowledge this system represents the first human brain-like tissue able to provide a more physiologically model for studying HCMV infection.
Pubmed
Web of science
Création de la notice
03/12/2015 15:30
Dernière modification de la notice
20/08/2019 15:18
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