Minute virus of mice non-structural protein NS-1 is necessary and sufficient for trans-activation of the viral P39 promoter.

Détails

ID Serval
serval:BIB_60C5A875FF51
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Minute virus of mice non-structural protein NS-1 is necessary and sufficient for trans-activation of the viral P39 promoter.
Périodique
The Journal of general virology
Auteur⸱e⸱s
Doerig C., Hirt B., Beard P., Antonietti J.P.
ISSN
0022-1317 (Print)
ISSN-L
0022-1317
Statut éditorial
Publié
Date de publication
10/1988
Peer-reviewed
Oui
Volume
69 ( Pt 10)
Pages
2563-2573
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The genome of the autonomous parvovirus minute virus of mice (MVM) is organized in two overlapping transcription units: the genes coding for the two non-structural proteins (NS-1 ad NS-2) are transcribed from a promoter (P04) located at map unit 4, whereas the promoter controlling the capsid protein genes (P39) lies at map unit 39. We studied the effect of viral proteins on the activity of the P39 promoter in vivo. By site-directed mutagenesis we constructed clones encoding only one of the two NS proteins. The activity of the P39 promoter was measured in HeLa or EL-4 cells transfected with these clones, either by an RNase protection assay or by following the expression of a reporter gene, CAT (which codes for chloramphenicol acetyltransferase), placed under the control of this promoter. We found that the P39 promoter of strain MVMi is activated in trans by a viral gene product, and evidence to suggest that NS-1 is the only viral gene product responsible for this trans-activation. We also determined that the mechanism of trans-activation is very rapid, since all species of viral mRNAs appear together in non-synchronized infected EL-4 cells within a 2 h interval.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Capsid/genetics, Cell Line, Chloramphenicol O-Acetyltransferase/genetics, Cloning, Molecular, Feedback, Gene Expression Regulation, Genes, Viral, Humans, Mice, Minute Virus of Mice/genetics, Molecular Sequence Data, Mutation, Nucleotide Mapping, Parvoviridae/genetics, Plasmids, Promoter Regions, Genetic, Proteins/genetics, RNA, Viral/biosynthesis, Time Factors, Transcription, Genetic, Transfection
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/06/2008 20:50
Dernière modification de la notice
14/04/2023 10:32
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