Molecular studies of the intestinal mucosal barrier physiopathology using cocultures of epithelial and immune cells: a technical update

Détails

ID Serval
serval:BIB_60B1F94F8599
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Molecular studies of the intestinal mucosal barrier physiopathology using cocultures of epithelial and immune cells: a technical update
Périodique
Microbes and Infection
Auteur⸱e⸱s
Kerneis  S., Caliot  E., Stubbe  H., Bogdanova  A., Kraehenbuhl  J., Pringault  E.
ISSN
1286-4579 (Print)
Statut éditorial
Publié
Date de publication
07/2000
Volume
2
Numéro
9
Pages
1119-24
Notes
Journal Article
Research Support, Non-U.S. Gov't
Review --- Old month value: Jul
Résumé
Peyer's patch lymphocytes cocultured with Caco-2 cells trigger the phenotypic conversion of enterocytes into cells that express morphological and functional M-cell properties. We report a technical update for setting up this model, which will enable the study of M-cell biology, the identification by biochemical approaches of molecules involved in the interaction of microorganisms with M cells, and the development of vectors that would efficiently target the mucosal immune system.
Mots-clé
Animals Bacterial Proteins/analysis Caco-2 Cells/*immunology Cell Differentiation Coculture Techniques/methods Enzyme-Linked Immunosorbent Assay Epithelial Cells/immunology Humans Mice Mice, Inbred BALB C Microscopy, Electron Peyer's Patches/*immunology Phagocytosis Ribosomal Proteins/analysis Vibrio cholerae
Pubmed
Web of science
Création de la notice
25/01/2008 15:05
Dernière modification de la notice
20/08/2019 14:18
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