In vitro differentiation of monocytoid THP-1 cells affects their permissiveness for HIV strains: a model system for studying the cellular basis of HIV differential tropism

Détails

ID Serval
serval:BIB_5FF7F06D2976
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vitro differentiation of monocytoid THP-1 cells affects their permissiveness for HIV strains: a model system for studying the cellular basis of HIV differential tropism
Périodique
Virology
Auteur(s)
Meylan  P. R., Spina  C. A., Richman  D. D., Kornbluth  R. S.
ISSN
0042-6822 (Print)
Statut éditorial
Publié
Date de publication
03/1993
Volume
193
Numéro
1
Pages
256-67
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Résumé
The prototypic macrophage-tropic HIV-1 isolate, HIV-1BaL, cannot replicate in the monocytoid cell line THP-1. After induction of differentiation by a phorbol diester, a fraction of THP-1 cells became permissive to HIV-1BaL. In contrast, this treatment decreased permissiveness for the lymphotropic isolate HIV-1LAI. Viral DNA was not synthesized in unstimulated THP-1 cells, as determined with PCR, suggesting that the block to HIV-1BaL replication in these cells occurred at an early step of the virus replicative cycle prior to or at the level of reverse transcription. Virus binding studies showed that differences in cell permissiveness for HIV-1BaL were not due to altered virus binding. Substantial amounts of HIV-1BaL bound to both undifferentiated and differentiated THP-1 cells, and this binding could not be prevented by blocking with the anti-CD4 antibody Leu3a, which did prevent the binding of HIV-1LAI to CEM T lymphoid cells. While Leu3a was very effective at preventing the infection by HIV-1LAI in CEM cells, it was less effective in preventing HIV-1BaL infection of differentiated THP-1 cells or primary macrophages. Although it is likely that molecules other than CD4 on monocytic cells can mediate binding of macrophage-tropic HIV, the binding of HIV-1BaL to THP-1 cells was not sufficient for infection, because binding was the same in nonpermissive undifferentiated cells as in permissive differentiated cells. Thus, the restriction of viral replication in this model cell system occurs at some step after virion binding. Comparison of differentiated THP-1 cells with their undifferentiated counterparts may provide an approach to defining cellular determinants of HIV host range other than CD4 expression and to characterizing the incompletely defined steps of viral entry.
Mots-clé
Antibodies, Monoclonal Antigens, CD4/physiology Cell Differentiation/drug effects/physiology DNA, Viral/biosynthesis HIV-1/*physiology Humans Macrophages/*microbiology Models, Biological Monocytes/cytology/*microbiology Tetradecanoylphorbol Acetate/pharmacology Tumor Cells, Cultured Virus Replication/*physiology
Pubmed
Web of science
Création de la notice
25/01/2008 15:32
Dernière modification de la notice
20/08/2019 15:17
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