Bioreporters: gfp versus lux revisited and single-cell response.

Détails

ID Serval
serval:BIB_5FD7B1735A6E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Bioreporters: gfp versus lux revisited and single-cell response.
Périodique
Biosensors and Bioelectronics
Auteur(s)
Kohlmeier S., Mancuso M., Tecon R., Harms H., van der Meer J.R., Wells M.
ISSN
0956-5663[print], 0956-5663[linking]
Statut éditorial
Publié
Date de publication
2007
Volume
22
Numéro
8
Pages
1578-1585
Langue
anglais
Résumé
Genetically engineered organisms expressing spectroscopically active reporter molecules in response to chemical effectors display great potential as living transducers in sensing applications. Green fluorescent protein (gfp gene) bioreporters have distinct advantages over luminescent couterparts (lux gene), including applicability at the single-cell level, but are typically less sensitive. Here we describe a gfp-bearing bioreporter that is sensitive to naphthalene (a poorly water soluble pollutant behaving like a large class of hydrophobic compounds), is suitable for use in chemical assays and bioavailability studies, and has detection limits comparable to lux-bearing bioreporters for higher efficiency detection strategies. Simultaneously, we find that the exploitation of population response data from single-cell analysis is not an algorithmic conduit to enhanced signal detection and hence lower effector detection limits, as normally assumed. The assay reported functions to equal effect with or without biocide.
Mots-clé
Biosensing Techniques/methods, Genes, Reporter, Green Fluorescent Proteins/genetics, Luciferases/genetics, Naphthalenes/toxicity
Pubmed
Web of science
Création de la notice
21/01/2008 14:35
Dernière modification de la notice
20/08/2019 15:17
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