Late neutrophil infiltration of canine endothelial cell seeded Dacron grafts

Détails

ID Serval
serval:BIB_5FB6EB1CF67C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Late neutrophil infiltration of canine endothelial cell seeded Dacron grafts
Périodique
ASAIO Transactions
Auteur⸱e⸱s
Muller-Glauser  W., Lachat  M., Baitella-Eberle  G., Neudecker  A., von Segesser  L. K., Turina  M., Moser  R.
ISSN
1058-2916
0889-7190
Statut éditorial
Publié
Date de publication
09/1990
Volume
36
Numéro
3
Pages
M763-6
Notes
Journal Article --- Old month value: Jul-Sep
Résumé
Neutrophil infiltration is known to affect the endothelial monolayer of seeded vascular grafts. The aim of this study was to develop an in vitro system allowing the monitoring of neutrophil (PMN) adherence after graft implantation. Dacron prostheses were seeded with autologous canine microvascular cells from omental adipose tissue and implanted for 35 days. In vitro, mature monolayers of canine homologous venous endothelial cells (CHVENC) were exposed to heparinized whole blood samples taken at days one and four postoperatively, followed by weekly tests. PMNs adherent to the CHVENC were counted per culture area. Results showed the feasibility of PMN monitoring, and demonstrated a late PMN adhesion, reaching its maximum about 20 days after implantation and decreasing to normal values after five weeks. It is concluded that in vitro tests can be used for noninvasive studies of host plasma factors and leukocyte activation.
Mots-clé
Animals *Blood Vessel Prosthesis Cell Adhesion/*physiology Dogs Endothelium, Vascular/*cytology Leukocyte Count Neutrophils/*cytology *Polyethylene Terephthalates Prosthesis Design Vascular Patency/physiology
Pubmed
Création de la notice
14/02/2008 15:17
Dernière modification de la notice
20/08/2019 15:17
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