Establishment of renal cell lines derived from S2 segments of the proximal tubule

Détails

ID Serval
serval:BIB_5F9E7B39833D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Establishment of renal cell lines derived from S2 segments of the proximal tubule
Périodique
Renal Physiology and Biochemistry
Auteur(s)
Fauth  C., Chabardes  D., Allaz  M., Garcia  M., Rossier  B., Roch-Ramel  F., Claire  M.
ISSN
1011-6524
Statut éditorial
Publié
Date de publication
06/1991
Volume
14
Numéro
3
Pages
128-39
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May-Jun
Résumé
The aim of this study was to establish epithelial cell lines derived from defined nephron segments. Primary cultures were prepared from dissected proximal S2 segments of the rabbit kidney, and grown in monolayers. Immortalization was observed after nuclear microinjection of the cells with simian virus 40 DNA and resulted in the development of cell lines of epithelial morphology. These cell lines were maintained in culture for at least 24 passages, then cells were frozen. One of the cell lines, the RKPC-2, was selected and further characterized. RKPC-2 cells formed domes on impermeable supports, indicating fluid and solute transport. RKPC-2 cells formed continuous monolayers of low transepithelial resistance on collagen-coated filters. They were able to accumulate tetraethylammonium, an organic cation; however, no significant transcellular transport could be measured. We conclude that this cell line which shows characteristics of epithelial cells has maintained certain properties of intact proximal tubules, in particular the capacity to accumulate organic cations.
Mots-clé
Animals Cell Division Cell Line *Cell Transformation, Neoplastic Culture Media Culture Techniques/methods DNA, Viral/analysis/genetics Kidney Tubules, Proximal/*cytology/physiology/ultrastructure Rabbits Simian virus 40/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 14:00
Dernière modification de la notice
20/08/2019 15:17
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