FANCM regulates DNA chain elongation and is stabilized by S-phase checkpoint signalling.

Détails

ID Serval
serval:BIB_5F815B15C01D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
FANCM regulates DNA chain elongation and is stabilized by S-phase checkpoint signalling.
Périodique
EMBO Journal
Auteur⸱e⸱s
Luke-Glaser S., Luke B., Grossi S., Constantinou A.
ISSN
1460-2075[electronic], 0261-4189[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
29
Numéro
4
Pages
795-805
Langue
anglais
Résumé
FANCM binds and remodels replication fork structures in vitro. We report that in vivo, FANCM controls DNA chain elongation in an ATPase-dependent manner. In the presence of replication inhibitors that do not damage DNA, FANCM counteracts fork movement, possibly by remodelling fork structures. Conversely, through damaged DNA, FANCM promotes replication and recovers stalled forks. Hence, the impact of FANCM on fork progression depends on the underlying hindrance. We further report that signalling through the checkpoint effector kinase Chk1 prevents FANCM from degradation by the proteasome after exposure to DNA damage. FANCM also acts in a feedback loop to stabilize Chk1. We propose that FANCM is a ringmaster in the response to replication stress by physically altering replication fork structures and by providing a tight link to S-phase checkpoint signalling.
Mots-clé
Adenosine Triphosphatases/metabolism, Base Sequence, DNA/biosynthesis, DNA/genetics, DNA Damage, DNA Helicases/antagonists & inhibitors, DNA Helicases/genetics, DNA Repair, DNA Replication/physiology, Fanconi Anemia Complementation Group D2 Protein/antagonists & inhibitors, Fanconi Anemia Complementation Group D2 Protein/genetics, Hela Cells, Humans, Models, Biological, Proteasome Endopeptidase Complex/metabolism, Protein Kinases/metabolism, RNA, Small Interfering/genetics, S Phase, Signal Transduction
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/09/2010 14:11
Dernière modification de la notice
20/08/2019 14:17
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