Evaluation of Mass Spectrometry-Based Detection of Panfungal Serum Disaccharide for Diagnosis of Invasive Fungal Infections: Results from a Collaborative Study Involving Six European Clinical Centers.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_5F7EBC190FD2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Evaluation of Mass Spectrometry-Based Detection of Panfungal Serum Disaccharide for Diagnosis of Invasive Fungal Infections: Results from a Collaborative Study Involving Six European Clinical Centers.
Périodique
Journal of clinical microbiology
Auteur⸱e⸱s
Cornu M., Sendid B., Mery A., François N., Mikulska M., Letscher-Bru V., De Carolis E., Damonti L., Titecat M., Bochud P.Y., Alanio A., Sanguinetti M., Viscoli C., Herbrecht R., Guerardel Y., Poulain D.
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Statut éditorial
Publié
Date de publication
05/2019
Peer-reviewed
Oui
Volume
57
Numéro
5
Langue
anglais
Notes
Publication types: Evaluation Study ; Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay. Patients with the following IFI were selected according to the availability of sera obtained at about the time that IFI was documented: invasive candidiasis (IC; n = 26 patients), invasive aspergillosis (IA; n = 19), and mucormycosis (MM; n = 23). Control sera originated from 20 neutropenic patients and 20 patients with bacteremia. MS-DS was carried out in blind manner for the diagnosis of IFI. A diagnosis of IC or IA was confirmed by detection of mannan (Man) or galactomannan (GM), respectively, associated with detection of (1,3)-β-d-glucan (BDG) in both infections. MM was detected by quantitative real-time PCR (qPCR). All tests discriminated sera from patients with IC from sera from control subjects with bacteremia (P ≤ 0.0009). For IC, the MS-DS sensitivity and specificity were 51% and 87%, respectively. MS-DS complemented the high specificity of Man monitoring. All tests discriminated sera from IA patients from sera from neutropenic controls (P ≤ 0.0009). For IA, MS-DS sensitivity and specificity were 64% and 95%, respectively. Only 13/36 serum samples from patients with MM were concordant by MS-DS and qPCR (6 were positive, and 7 were negative); 14 were positive by MS-DS alone. qPCR and MS-DS made a similar contribution to the diagnosis of MM. In patients undergoing long-term monitoring, the persistent circulation of serum disaccharide was observed, whereas DNA was detected only for a short period after initiation of treatment. MS-DS has an important role to play in the early diagnosis of IFI. Its panfungal nature and complementarity with other tests may justify its use in the management of IFI.
Mots-clé
Adult, Aged, Aged, 80 and over, Antigens, Fungal/blood, Aspergillosis/diagnosis, Candidiasis/diagnosis, Disaccharides/blood, Europe, Female, Humans, Intersectoral Collaboration, Invasive Fungal Infections/blood, Invasive Fungal Infections/diagnosis, Male, Mannans/blood, Mass Spectrometry, Middle Aged, Mucormycosis/diagnosis, Sensitivity and Specificity, Young Adult, invasive aspergillosis, invasive candidiasis, invasive fungal infection, mass spectrometry, mucormycosis, serological diagnosis
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/04/2019 16:28
Dernière modification de la notice
13/01/2021 7:09
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