Purification of potato leaf plasma membrane protein PP34, a protein phosphorylated in response to oligogalacturonide signals for defense and development.

Détails

ID Serval
serval:BIB_5F2788C2B4BF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Purification of potato leaf plasma membrane protein PP34, a protein phosphorylated in response to oligogalacturonide signals for defense and development.
Périodique
Plant Physiology
Auteur(s)
Jacinto T., Farmer E.E., Ryan C.A.
ISSN
1532-2548 (Electronic)
ISSN-L
0032-0889
Statut éditorial
Publié
Date de publication
1993
Volume
103
Numéro
4
Pages
1393-1397
Langue
anglais
Résumé
A potato (Solanum tuberosum L.) plasma membrane protein called pp34, the only known example of a plasma membrane protein that is phosphorylated specifically in response to defined Oligogalacturonide signals in plants, has been purified to apparent homogeneity. Polyclonal antibodies raised in rabbits against the purified pp34 protein immunoprecipitated a single thiophosphorylated protein species from potato plasma membranes, as analyzed by two-dimensional denaturing electrophoresis and fluorography. The pp34 antibodies also recognized a single protein in tomato (Lycopersicon esculentum L.) membranes that is thiophosphorylated in response to Oligogalacturonide elicitors, as demonstrated by western blotting and specific immunoprecipitation. These experiments confirm the identity of the tomato membrane protein as a pp34 homolog and establish the high monospecificity of the pp34 antibodies. This will permit further investigation of the role of protein phosphorylation in oligouronide signaling for defensive genes in potato and tomato plants.
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 21:05
Dernière modification de la notice
20/08/2019 15:16
Données d'usage