Axial and Radial Oxylipin Transport.

Détails

ID Serval
serval:BIB_5EA4FBA8A845
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Axial and Radial Oxylipin Transport.
Périodique
Plant physiology
Auteur(s)
Gasperini D., Chauvin A., Acosta I.F., Kurenda A., Stolz S., Chételat A., Wolfender J.L., Farmer E.E.
ISSN
1532-2548 (Electronic)
ISSN-L
0032-0889
Statut éditorial
Publié
Date de publication
11/2015
Peer-reviewed
Oui
Volume
169
Numéro
3
Pages
2244-2254
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Jasmonates are oxygenated lipids (oxylipins) that control defense gene expression in response to cell damage in plants. How mobile are these potent mediators within tissues? Exploiting a series of 13-lipoxygenase (13-lox) mutants in Arabidopsis (Arabidopsis thaliana) that displays impaired jasmonic acid (JA) synthesis in specific cell types and using JA-inducible reporters, we mapped the extent of the transport of endogenous jasmonates across the plant vegetative growth phase. In seedlings, we found that jasmonate (or JA precursors) could translocate axially from wounded shoots to unwounded roots in a LOX2-dependent manner. Grafting experiments with the wild type and JA-deficient mutants confirmed shoot-to-root oxylipin transport. Next, we used rosettes to investigate radial cell-to-cell transport of jasmonates. After finding that the LOX6 protein localized to xylem contact cells was not wound inducible, we used the lox234 triple mutant to genetically isolate LOX6 as the only JA precursor-producing LOX in the plant. When a leaf of this mutant was wounded, the JA reporter gene was expressed in distal leaves. Leaf sectioning showed that JA reporter expression extended from contact cells throughout the vascular bundle and into extravascular cells, revealing a radial movement of jasmonates. Our results add a crucial element to a growing picture of how the distal wound response is regulated in rosettes, showing that both axial (shoot-to-root) and radial (cell-to-cell) transport of oxylipins plays a major role in the wound response. The strategies developed herein provide unique tools with which to identify intercellular jasmonate transport routes.
Mots-clé
Arabidopsis/enzymology, Arabidopsis/genetics, Arabidopsis/physiology, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Biological Transport, Cyclopentanes/metabolism, Gene Expression Regulation, Plant, Lipoxygenase/genetics, Lipoxygenase/metabolism, Lipoxygenases/genetics, Lipoxygenases/metabolism, Oxylipins/metabolism, Plant Growth Regulators/metabolism, Plant Leaves/enzymology, Plant Leaves/genetics, Plant Leaves/physiology, Plant Roots/enzymology, Plant Roots/genetics, Plant Roots/physiology, Plant Shoots/enzymology, Plant Shoots/genetics, Plant Shoots/physiology, Seedlings/enzymology, Seedlings/genetics, Seedlings/physiology, Stress, Physiological, Xylem/enzymology, Xylem/genetics, Xylem/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/07/2016 9:19
Dernière modification de la notice
15/07/2020 6:26
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