Enhanced expression of three monocarboxylate transporter isoforms in the brain of obese mice.

Détails

ID Serval
serval:BIB_5E9864405ABC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Enhanced expression of three monocarboxylate transporter isoforms in the brain of obese mice.
Périodique
Journal of Physiology
Auteur(s)
Pierre K., Parent A., Jayet P.Y., Halestrap A.P., Scherrer U., Pellerin L.
ISSN
0022-3751
Statut éditorial
Publié
Date de publication
09/2007
Peer-reviewed
Oui
Volume
583
Numéro
Pt 2
Pages
469-486
Langue
anglais
Résumé
Monocarboxylate transporters (MCTs) are membrane carriers for lactate and ketone bodies. Three isoforms, MCT1, MCT2 and MCT4, have been described in the central nervous system but little information is available about the regulation of their expression in relation to altered metabolic and/or nutritional conditions. We show here that brains of mice fed on a high fat diet (HFD) up to 12 weeks as well as brains of genetically obese (ob/ob) or diabetic (db/db) mice exhibit an increase of MCT1, MCT2 and MCT4 expression as compared to brains of control mice fed a standard diet. Enhanced expression of each transporter was visible throughout the brain but most prominently in the cortex and in the hippocampus. Using immunohistochemistry, we observed that neurons (expressing mainly MCT2 but also sometimes low levels of MCT1 under normal conditions) were immunolabelled for all three transporters in HFD mice as well as in ob/ob and db/db mice. At the subcellular level, changes were most remarkable in neuronal cell bodies. Western blotting performed on brain structure extracts allowed us to confirm quantitatively the enhancement of MCT1 and MCT2 expression. Our data demonstrate that the expression of cerebral MCT isoforms can be modulated by alterations of peripheral metabolism, suggesting that the adult brain is sensitive and adapts to new metabolic states. This observation could be relevant in the context of obesity development and its consequences for brain function.
Mots-clé
Animals, Blotting, Western, Brain/metabolism, Brain/pathology, Cerebellum/metabolism, Cerebral Cortex/metabolism, Dietary Fats/adverse effects, Hippocampus/metabolism, Hyperglycemia/genetics, Hyperglycemia/metabolism, Hyperinsulinism/genetics, Hyperinsulinism/metabolism, Immunohistochemistry, Male, Mice, Mice, Inbred C57BL, Mice, Obese, Monocarboxylic Acid Transporters/metabolism, Muscle Proteins/metabolism, Neurons/metabolism, Obesity/etiology, Obesity/genetics, Symporters/metabolism, Time Factors, Up-Regulation
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:17
Dernière modification de la notice
20/08/2019 15:16
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