Three-dimensional Ca2+ imaging advances understanding of astrocyte biology.

Détails

Ressource 1Télécharger: 28522470_Author_manuscript.pdf (11341.40 [Ko])
Etat: Public
Version: Author's accepted manuscript
Document(s) secondaire(s)
Télécharger: aai8185_Bindocci_SM.pdf (13582.37 [Ko])
Etat: Public
Version: Supplementary document
Télécharger: aai8185s1.mp4 (3190.74 [Ko])
Etat: Public
Version: Supplementary document
Télécharger: aai8185s2.mp4 (6185.69 [Ko])
Etat: Public
Version: Supplementary document
Télécharger: aai8185s3.mp4 (8392.72 [Ko])
Etat: Public
Version: Supplementary document
Télécharger: aai8185s4.mp4 (8412.22 [Ko])
Etat: Public
Version: Supplementary document
ID Serval
serval:BIB_5E81DDD61A2E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Three-dimensional Ca2+ imaging advances understanding of astrocyte biology.
Périodique
Science
Auteur(s)
Bindocci E., Savtchouk I., Liaudet N., Becker D., Carriero G., Volterra A.
ISSN
1095-9203 (Electronic)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
19/05/2017
Peer-reviewed
Oui
Volume
356
Numéro
6339
Pages
0
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Astrocyte communication is typically studied by two-dimensional calcium ion (Ca2+) imaging, but this method has not yielded conclusive data on the role of astrocytes in synaptic and vascular function. We developed a three-dimensional two-photon imaging approach and studied Ca2+ dynamics in entire astrocyte volumes, including during axon-astrocyte interactions. In both awake mice and brain slices, we found that Ca2+ activity in an individual astrocyte is scattered throughout the cell, largely compartmented between regions, preponderantly local within regions, and heterogeneously distributed regionally and locally. Processes and endfeet displayed frequent fast activity, whereas the soma was infrequently active. In awake mice, activity was higher than in brain slices, particularly in endfeet and processes, and displayed occasional multifocal cellwide events. Astrocytes responded locally to minimal axonal firing with time-correlated Ca2+ spots.

Mots-clé
Animals, Astrocytes/cytology, Astrocytes/metabolism, Axons/metabolism, Calcium Signaling, Hippocampus/cytology, Imaging, Three-Dimensional, Mice, Neuroanatomical Tract-Tracing Techniques, Wakefulness
Pubmed
Web of science
Création de la notice
30/05/2017 17:43
Dernière modification de la notice
20/08/2019 15:16
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