Diethyl pyrocarbonate: a chemical probe for secondary structure in negatively supercoiled DNA.

Détails

ID Serval
serval:BIB_5E45E3FF13DC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Diethyl pyrocarbonate: a chemical probe for secondary structure in negatively supercoiled DNA.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Herr W.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
1985
Volume
82
Numéro
23
Pages
8009-8013
Langue
anglais
Résumé
Purine residues located within regions of DNA that have the potential to form left-handed Z-helical structures are modified preferentially by diethyl pyrocarbonate; this hyperreactivity is dependent on the degree of negative superhelicity of the circular DNA molecules. As negative superhelical density increases, guanosines in a 32-base-pair alternating G-C sequence and adenosines (but not guanosines) in a 64-base-pair alternating A-C/G-T sequence become 5- to 10-fold more reactive to diethyl pyrocarbonate. The negative superhelical densities at which enhanced reactivity occurs are similar to those reported for the point at which left-handed helices form within plasmids carrying these DNA sequences. Probing of negatively supercoiled pBR322 with diethyl pyrocarbonate reveals a hyperreactive region 31 base pairs in length of which only 9 base pairs are a perfect alternating purine and pyrimidine sequence; the reactivity of purines within this sequence indicates that purines in the anti conformation, or guanosines in the syn conformation with neighboring 3' thymidines, are not hyperreactive in the Z-DNA form.
Mots-clé
Adenosine, Alkylation, Base Sequence, DNA, DNA, Superhelical, Diethyl Pyrocarbonate/diagnostic use, Formates/diagnostic use, Guanosine, Nucleic Acid Conformation, Purines, Structure-Activity Relationship
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
20/08/2019 15:16
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