Retention of the differentiated state by larval Xenopus liver cells in primary culture

Détails

ID Serval
serval:BIB_5DCD5A99B9CB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Retention of the differentiated state by larval Xenopus liver cells in primary culture
Périodique
Wilhelm Roux's Archives of Developmental Biology
Auteur⸱e⸱s
Wahli W., Abraham I., Weber R.
ISSN
0949-944X
0930-035X
Statut éditorial
Publié
Date de publication
1978
Volume
185
Numéro
3
Pages
235-248
Langue
anglais
Résumé
Electron microscopic analysis of primary cultures derived from larvalXenopus liver has shown that these cells, although they form only two-dimensional aggregates, retain and presumably also develop structural characteristics typical of liver parenchyma cells, such as bile canaliculi with microvilli and epithelial junctional complexes. As judged from structural criteria, primary cultures contain 80-90% hepatocytes. In contrast to the intact tissue, primary cultures showed excessive development of microfilaments, however.
Incorporation of labeled amino acids has revealed further that the capacity for protein synthesis is maintained in culture and that synthesis of liverspecific protein albumin is maintained in vitro, even in liver cultures derived from thyrostatic tadpoles. This latter result suggests that initiation of albumin synthesis in the larval liver is probably not dependent upon thyroid hormones but rather reflects the protodifferentiated state of this tissue.
Mots-clé
Liver, Primary culture, Ultrastructure, Albumin synthesis, Xenopus
Création de la notice
25/03/2010 11:38
Dernière modification de la notice
20/08/2019 14:15
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