Makorin ring zinc finger protein 1 (MKRN1), a novel poly(A)-binding protein-interacting protein, stimulates translation in nerve cells.

Détails

ID Serval
serval:BIB_5D3554930B64
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Makorin ring zinc finger protein 1 (MKRN1), a novel poly(A)-binding protein-interacting protein, stimulates translation in nerve cells.
Périodique
The Journal of biological chemistry
Auteur(s)
Miroci H., Schob C., Kindler S., Ölschläger-Schütt J., Fehr S., Jungenitz T., Schwarzacher S.W., Bagni C., Mohr E.
ISSN
1083-351X (Electronic)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
06/01/2012
Volume
287
Numéro
2
Pages
1322-1334
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The poly(A)-binding protein (PABP), a key component of different ribonucleoprotein complexes, plays a crucial role in the control of mRNA translation rates, stability, and subcellular targeting. In this study we identify RING zinc finger protein Makorin 1 (MKRN1), a bona fide RNA-binding protein, as a binding partner of PABP that interacts with PABP in an RNA-independent manner. In rat brain, a so far uncharacterized short MKRN1 isoform, MKRN1-short, predominates and is detected in forebrain nerve cells. In neuronal dendrites, MKRN1-short co-localizes with PABP in granule-like structures, which are morphological correlates of sites of mRNA metabolism. Moreover, in primary rat neurons MKRN1-short associates with dendritically localized mRNAs. When tethered to a reporter mRNA, MKRN1-short significantly enhances reporter protein synthesis. Furthermore, after induction of synaptic plasticity via electrical stimulation of the perforant path in vivo, MKRN1-short specifically accumulates in the activated dendritic lamina, the middle molecular layer of the hippocampal dentate gyrus. Collectively, these data indicate that in mammalian neurons MKRN1-short interacts with PABP to locally control the translation of dendritic mRNAs at synapses.

Mots-clé
Animals, Dendrites/genetics, Dendrites/metabolism, Dentate Gyrus/cytology, Dentate Gyrus/metabolism, Male, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, Neuronal Plasticity/physiology, Poly(A)-Binding Proteins/genetics, Poly(A)-Binding Proteins/metabolism, Protein Biosynthesis/physiology, Protein Isoforms/genetics, Protein Isoforms/metabolism, RNA, Messenger/genetics, RNA, Messenger/metabolism, Rats, Rats, Sprague-Dawley, Synapses/genetics, Synapses/metabolism
Pubmed
Open Access
Oui
Création de la notice
06/03/2017 18:23
Dernière modification de la notice
20/08/2019 15:15
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