A TARBP2-dependent miRNA expression profile underlies cancer stem cell properties and provides candidate therapeutic reagents in Ewing sarcoma.

Détails

ID Serval
serval:BIB_5D2E37405825
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A TARBP2-dependent miRNA expression profile underlies cancer stem cell properties and provides candidate therapeutic reagents in Ewing sarcoma.
Périodique
Cancer cell
Auteur(s)
De Vito C., Riggi N., Cornaz S., Suvà M.L., Baumer K., Provero P., Stamenkovic I.
ISSN
1878-3686 (Electronic)
ISSN-L
1535-6108
Statut éditorial
Publié
Date de publication
12/06/2012
Peer-reviewed
Oui
Volume
21
Numéro
6
Pages
807-821
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
We have recently demonstrated that human pediatric mesenchymal stem cells can be reprogrammed toward a Ewing sarcoma family tumor (ESFT) cancer stem cell (CSC) phenotype by mechanisms that implicate microRNAs (miRNAs). Here, we show that the miRNA profile of ESFT CSCs is shared by embryonic stem cells and CSCs from divergent tumor types. We also provide evidence that the miRNA profile of ESFT CSCs is the result of reversible disruption of TARBP2-dependent miRNA maturation. Restoration of TARBP2 activity and systemic delivery of synthetic forms of either of two of its targets, miRNA-143 or miRNA-145, inhibited ESFT CSC clonogenicity and tumor growth in vivo. Our observations suggest that CSC self-renewal and tumor maintenance may depend on deregulation of TARBP2-dependent miRNA expression.
Mots-clé
Animals, Cell Line, Tumor, Cells, Cultured, Child, Child, Preschool, Embryonic Stem Cells/metabolism, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Induced Pluripotent Stem Cells/metabolism, Male, Mesenchymal Stromal Cells/metabolism, Mice, Mice, Inbred NOD, Mice, Knockout, Mice, SCID, MicroRNAs/genetics, Mutation, Neoplasms, Experimental/genetics, Neoplasms, Experimental/metabolism, Neoplasms, Experimental/pathology, Neoplastic Stem Cells/metabolism, Neoplastic Stem Cells/pathology, RNA-Binding Proteins/genetics, RNA-Binding Proteins/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sarcoma, Ewing/genetics, Sarcoma, Ewing/pathology, Sarcoma, Ewing/therapy, Transplantation, Heterologous
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/06/2012 9:56
Dernière modification de la notice
20/08/2019 15:15
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