Luteogenic hormones act through a vascular endothelial growth factor-dependent mechanism to up-regulate alpha 5 beta 1 and alpha v beta 3 integrins, promoting the migration and survival of human luteinized granulosa cells.

Détails

ID Serval
serval:BIB_5CDF6014A503
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Luteogenic hormones act through a vascular endothelial growth factor-dependent mechanism to up-regulate alpha 5 beta 1 and alpha v beta 3 integrins, promoting the migration and survival of human luteinized granulosa cells.
Périodique
American Journal of Pathology
Auteur(s)
Rolaki A., Coukos G., Loutradis D., DeLisser H.M., Coutifaris C., Makrigiannakis A.
ISSN
0002-9440 (Print)
ISSN-L
0002-9440
Statut éditorial
Publié
Date de publication
2007
Volume
170
Numéro
5
Pages
1561-1572
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
The formation of the corpus luteum (CL) is critical for the establishment of a successful pregnancy. After ovulation, the CL develops from the remnants of the ovulated ovarian follicle. This process, which involves varying cell-matrix interactions, is poorly characterized. To understand the role and potential regulation of cell-matrix interactions in the formation of the CL, we investigated the expression and activity of the matrix protein fibronectin (FN) and several of its integrin receptors on luteinized granulosa cells (GCs). In situ, FN and several FN-binding integrins were detected around luteinizing GCs during the early luteal phase, although expression declined in the late luteal phase. In vitro, GCs released FN, and stimulation of these cells with human chorionic gonadotropin increased the surface expression of FN, alpha(5)beta(1), and alpha(v)beta(3). Up-regulation of these proteins on GCs was reproduced by stimulation with vascular endothelial growth factor (VEGF) and was inhibited by anti-VEGF antibody. Lastly, expression of alpha(5)beta(1) and alpha(v)beta(3) mediated adhesion to FN, facilitated migration, and prevented apoptosis. These data suggest that in vivo luteogenic hormones, in part through a VEGF-dependent mechanism, stimulate selected integrin-matrix adhesive interactions that promote the motility and survival of GCs and thus contribute to the formation and preservation of the CL.
Mots-clé
Adult, Apoptosis, Cell Adhesion/physiology, Cell Movement/physiology, Cell Survival/physiology, Cells, Cultured, Chorionic Gonadotropin/metabolism, Female, Fertility Agents, Female/pharmacology, Fibronectins/metabolism, Flow Cytometry, Fluorescent Antibody Technique, Follicle Stimulating Hormone/pharmacology, Gonadotropins, Pituitary/metabolism, Granulosa Cells/metabolism, Humans, Immunohistochemistry, In Situ Nick-End Labeling, Integrin alpha5/metabolism, Leuprolide/pharmacology, Menstrual Cycle/physiology, Pregnancy, Up-Regulation, Vascular Endothelial Growth Factor A/metabolism
Pubmed
Web of science
Création de la notice
14/10/2014 11:42
Dernière modification de la notice
20/08/2019 14:15
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