Symmetrical arrangement of the heterologous regions of rabbit poxvirus and vaccinia virus DNA.

Détails

ID Serval
serval:BIB_5CD963A31FB1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Symmetrical arrangement of the heterologous regions of rabbit poxvirus and vaccinia virus DNA.
Périodique
Journal of General Virology
Auteur⸱e⸱s
Schümperli D., Menna A., Schwendimann F., Wittek R., Wyler R.
ISSN
0022-1317[print], 0022-1317[linking]
Statut éditorial
Publié
Date de publication
04/1980
Volume
47
Numéro
2
Pages
385-398
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Résumé
Cleavage sites for the restriction endonucleases EcoRI, KpnI and XhoI were mapped on rabbit poxvirus and vaccinia virus DNA. These physical maps were used to analyse the structural variations between the two DNAs. Two specific heterologous regions, symmetrically arranged at each end of the genomes, have been identified. Region 1, representing the exterior part of the terminal repetition, appears to contain unrelated sequences in each DNA and accounts for the difference in length of the two genomes. Region 2, separated from region 1 by a conserved part of the terminal repetition, is located at the transition from repeated to unique DNA sequences. Its overall length of about 4 megadaltons is well conserved and it contains individual DNA-specific as well as conserved restriction sites. The major central part of the genomes (over 100 megadaltons) contains very few, widely dispersed restriction site variations.
Mots-clé
Base Sequence, DNA Restriction Enzymes, DNA, Viral/analysis, Electrophoresis, Agar Gel, Genes, Viral, Poxviridae/analysis, Vaccinia virus/analysis, Vaccinia virus/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 10:43
Dernière modification de la notice
20/08/2019 14:15
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