Recombinant fusion peptides containing single or multiple repeats of a ubiquitous T-helper epitope are highly immunogenic

Détails

ID Serval
serval:BIB_5CB767106B90
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Recombinant fusion peptides containing single or multiple repeats of a ubiquitous T-helper epitope are highly immunogenic
Périodique
Molecular Immunology
Auteur(s)
Astori  M., Kraehenbuhl  J. P.
ISSN
0161-5890 (Print)
Statut éditorial
Publié
Date de publication
09/1996
Volume
33
Numéro
13
Pages
1017-24
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
Two recombinant mouse mammary tumor virus (MMTV) subunit vaccines have been constructed, in which linear sequences of the envelope gp 52 glycoprotein (EP3) or the superantigen (SAg) have been fused to single or multiple repeats of a T-cell epitope (P30) from tetanus toxin. Histidine tags or glutathione-S-transferase (GST) sequences have been included in the recombinant peptides in order to facilitate their purification by affinity chromatography. The EP3 or SAg recombinant polypeptides with four, one, or no T-cell epitopes were expressed in Escherichia coli, purified and injected intramuscularly, with non-ionic block copolymers as an adjuvant, into BALB/c mice. Following one or two boosts, the B- and T-cell responses against the recombinant proteins were analysed. The addition of T-cell epitopes considerably increased the immunogenicity of the subunit vaccines. The anti-EP3 response was maximum with four T-cell epitopes, while a single T epitope was optimal for the anti-SAg response.
Mots-clé
Animals Antibodies, Viral/blood Antigens, Viral/*immunology Antigens, Viral, Tumor/genetics/immunology Epitopes/genetics/*immunology Female Mammary Tumor Virus, Mouse/*immunology Mice Mice, Inbred BALB C Recombinant Fusion Proteins/immunology Repetitive Sequences, Nucleic Acid Superantigens/genetics/immunology T-Lymphocytes, Helper-Inducer/*immunology Tetanus Toxin/immunology Vaccination Vaccines, Synthetic Viral Vaccines/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
20/08/2019 15:15
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