Sodium sensing in neurons with a dendrimer-based nanoprobe.

Détails

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Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_5CAB550B923C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Sodium sensing in neurons with a dendrimer-based nanoprobe.
Périodique
ACS Nano
Auteur(s)
Lamy C.M., Sallin O., Loussert C., Chatton J.Y.
ISSN
1936-086X (Electronic)
ISSN-L
1936-0851
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
6
Numéro
2
Pages
1176-1187
Langue
anglais
Résumé
Ion imaging is a powerful methodology to assess fundamental biological processes in live cells. The limited efficiency of some ion-sensing probes and their fast leakage from cells are important restrictions to this approach. In this study, we present a novel strategy based on the use of dendrimer nanoparticles to obtain better intracellular retention of fluorescent probes and perform prolonged fluorescence imaging of intracellular ion dynamics. A new sodium-sensitive nanoprobe was generated by encapsulating a sodium dye in a PAMAM dendrimer nanocontainer. This nanoprobe is very stable and has high sodium sensitivity and selectivity. When loaded in neurons in live brain tissue, it homogenously fills the entire cell volume, including small processes, and stays for long durations, with no detectable alterations of cell functional properties. We demonstrate the suitability of this new sodium nanosensor for monitoring physiological sodium responses such as those occurring during neuronal activity.
Mots-clé
PAMAM dendrimer, nanoprobe, fluorescence imaging, neuron, sodium
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/03/2012 17:07
Dernière modification de la notice
20/08/2019 15:15
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