Germinal center dendritic cells express more ICAM-1 than extrafollicular dendritic cells and ICAM-1/LFA-1 interactions are involved in the capacity of dendritic cells to induce PBMCs proliferation.
Détails
ID Serval
serval:BIB_5C8D342CDAB5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Germinal center dendritic cells express more ICAM-1 than extrafollicular dendritic cells and ICAM-1/LFA-1 interactions are involved in the capacity of dendritic cells to induce PBMCs proliferation.
Périodique
Journal of Histochemistry and Cytochemistry
ISSN
0022-1554[print], 0022-1554[linking]
Statut éditorial
Publié
Date de publication
2006
Volume
54
Numéro
1
Pages
75-84
Langue
anglais
Résumé
Germinal center dendritic cells (GCDCs) have been identified as CD11c(+) CD4(+) CD3(-) cells located in GCs with the ability of inducing marked proliferation of allogenic T cells. Using immunofluorescence techniques, we have observed that this CD11c(+) CD4(+) CD3(-) immunophenotype identified GCDCs but also a subset of extrafollicular DCs. By flow cytometry, we were able to discriminate the GCDCs (CD11c(high) CD4(high) lin(-)) from the other tonsil DCs. By immunofluorescence and flow cytometry, we found that dendritic cells of germinal centers express more intracellular adhesion molecule-1 (ICAM-1) (CD54) than extrafollicular dendritic cells. Proliferation of peripheral blood mononuclear cells (PBMCs) induced by coculture with purified CD11c(+) CD4(+) CD3(-) DCs was reduced by addition of blocking anti-CD54 antibodies. In summary, distinct levels of ICAM-1 expression allow the distinction between GCDCs and extrafollicular DCs, and cellular interactions mediated by CD54 are likely to play a role in the capacity of GCDC to stimulate allogenic PBMC proliferation.
Mots-clé
Antigens, CD11c/metabolism, Antigens, CD3/metabolism, Antigens, CD4/metabolism, Cell Proliferation, Coculture Techniques, Dendritic Cells/cytology, Dendritic Cells/metabolism, Flow Cytometry, Fluorescent Antibody Technique, Germinal Center/cytology, Germinal Center/metabolism, Humans, Immunophenotyping, Intercellular Adhesion Molecule-1/metabolism, Leukocytes, Mononuclear/physiology, Lymphocyte Function-Associated Antigen-1/metabolism
Pubmed
Création de la notice
28/10/2010 10:40
Dernière modification de la notice
20/08/2019 15:15