Selective accumulation of differentiated FOXP3(+) CD4 (+) T cells in metastatic tumor lesions from melanoma patients compared to peripheral blood

Détails

Ressource 1Télécharger: serval:BIB_5C81771B51CC.P001 (577.70 [Ko])
Etat: Public
Version: de l'auteur
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ID Serval
serval:BIB_5C81771B51CC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Selective accumulation of differentiated FOXP3(+) CD4 (+) T cells in metastatic tumor lesions from melanoma patients compared to peripheral blood
Périodique
Cancer Immunology, Immunotherapy
Auteur(s)
Jandus C., Bioley G., Speiser D.E., Romero P.
ISSN
0340-7004
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
57
Numéro
12
Pages
1795-1805
Langue
anglais
Résumé
Precise identification of regulatory T cells is crucial in the understanding of their role in human cancers. Here, we analyzed the frequency and phenotype of regulatory T cells (Tregs), in both healthy donors and melanoma patients, based on the expression of the transcription factor FOXP3, which, to date, is the most reliable marker for Tregs, at least in mice. We observed that FOXP3 expression is not confined to human CD25(+/high) CD4(+) T cells, and that these cells are not homogenously FOXP3(+). The circulating relative levels of FOXP3(+) CD4(+) T cells may fluctuate close to 2-fold over a short period of observation and are significantly higher in women than in men. Further, we showed that FOXP3(+) CD4(+) T cells are over-represented in peripheral blood of melanoma patients, as compared to healthy donors, and that they are even more enriched in tumor-infiltrated lymph nodes and at tumor sites, but not in normal lymph nodes. Interestingly, in melanoma patients, a significantly higher proportion of functional, antigen-experienced FOXP3(+) CD4(+) T was observed at tumor sites, compared to peripheral blood. Together, our data suggest that local accumulation and differentiation of Tregs is, at least in part, tumor-driven, and illustrate a reliable combination of markers for their monitoring in various clinical settings.
Mots-clé
Cell Differentiation, Female, Flow Cytometry, Forkhead Transcription Factors, Humans, Lymph Nodes, Lymphatic Metastasis, Male, Melanoma, Middle Aged, T-Lymphocytes, Regulatory
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/01/2009 13:09
Dernière modification de la notice
01/10/2019 7:18
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