Combination of fluorescence microscopy and nanomotion detection to characterize bacteria.

Détails

ID Serval
serval:BIB_5C6B1759AEF2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Combination of fluorescence microscopy and nanomotion detection to characterize bacteria.
Périodique
Journal of Molecular Recognition
Auteur⸱e⸱s
Aghayee S., Benadiba C., Notz J., Kasas S., Dietler G., Longo G.
ISSN
1099-1352 (Electronic)
ISSN-L
0952-3499
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
26
Numéro
11
Pages
590-595
Langue
anglais
Résumé
Antibiotic-resistant pathogens are a major health concern in everyday clinical practice. Because their detection by conventional microbial techniques requires minimally 24 h, some of us have recently introduced a nanomechanical sensor, which can reveal motion at the nanoscale. By monitoring the fluctuations of the sensor, this technique can evidence the presence of bacteria and their susceptibility to antibiotics in less than 1 h. Their amplitude correlates to the metabolism of the bacteria and is a powerful tool to characterize these microorganisms at low densities. This technique is new and calls for an effort to optimize its protocol and determine its limits. Indeed, many questions remain unanswered, such as the detection limits or the correlation between the bacterial distribution on the sensor and the detection's output. In this work, we couple fluorescence microscopy to the nanomotion investigation to determine the optimal experimental protocols and to highlight the effect of the different bacterial distributions on the sensor.
Mots-clé
Nanomechanical sensors, Fluorescence microscopy, Bacteria, Nanomotion detector, metabolism, AFM
Pubmed
Web of science
Création de la notice
20/12/2013 14:00
Dernière modification de la notice
20/08/2019 15:14
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