Genome-wide analysis of cancer/testis gene expression.

Détails

ID Serval
serval:BIB_5C5775EFF0D9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Genome-wide analysis of cancer/testis gene expression.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Hofmann O., Caballero O.L., Stevenson B.J., Chen Y.T., Cohen T., Chua R., Maher C.A., Panji S., Schaefer U., Kruger A., Lehvaslaiho M., Carninci P., Hayashizaki Y., Jongeneel C.V., Simpson A.J., Old L.J., Hide W.
ISSN
1091-6490[electronic], 0027-8424[linking]
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
105
Numéro
51
Pages
20422-20427
Langue
anglais
Résumé
Cancer/Testis (CT) genes, normally expressed in germ line cells but also activated in a wide range of cancer types, often encode antigens that are immunogenic in cancer patients, and present potential for use as biomarkers and targets for immunotherapy. Using multiple in silico gene expression analysis technologies, including twice the number of expressed sequence tags used in previous studies, we have performed a comprehensive genome-wide survey of expression for a set of 153 previously described CT genes in normal and cancer expression libraries. We find that although they are generally highly expressed in testis, these genes exhibit heterogeneous gene expression profiles, allowing their classification into testis-restricted (39), testis/brain-restricted (14), and a testis-selective (85) group of genes that show additional expression in somatic tissues. The chromosomal distribution of these genes confirmed the previously observed dominance of X chromosome location, with CT-X genes being significantly more testis-restricted than non-X CT. Applying this core classification in a genome-wide survey we identified >30 CT candidate genes; 3 of them, PEPP-2, OTOA, and AKAP4, were confirmed as testis-restricted or testis-selective using RT-PCR, with variable expression frequencies observed in a panel of cancer cell lines. Our classification provides an objective ranking for potential CT genes, which is useful in guiding further identification and characterization of these potentially important diagnostic and therapeutic targets.
Mots-clé
A Kinase Anchor Proteins, Cell Line, Tumor, Chromosomes, Human, Chromosomes, Human, X, Computational Biology, GPI-Linked Proteins, Gene Expression Profiling/methods, Genome, Human, Genomics/methods, Homeodomain Proteins/genetics, Humans, Male, Membrane Proteins/genetics, Testicular Neoplasms/genetics, Testis
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/01/2011 10:32
Dernière modification de la notice
20/08/2019 14:14
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