Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes.

Détails

ID Serval
serval:BIB_5C4E1A628682
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes.
Périodique
Journal of Assisted Reproduction and Genetics
Auteur(s)
Chanson A., Nocera D., Senn A., De Grandi P., Germond M.
ISSN
1058-0468 (Print)
ISSN-L
1058-0468
Statut éditorial
Publié
Date de publication
2001
Volume
18
Numéro
2
Pages
97-105
Langue
anglais
Résumé
PURPOSE: Our purpose was to develop a well-defined medium for the in vitro maturation (IVM) of immature bovine cumulus-oocyte complexes (COC).
METHODS: The COC were cultured in the presence of three protein supplementations: fetal bovine serum (FBS), bovine serum albumin, and Synthetic Serum Substitute. The embryos obtained after in vitro fertilization of IVM oocytes were cocultured with Vero cells and their development to the morula and blastocyst stages was studied.
RESULTS: When FBS was absent from the IVM medium, a significantly lower fertilization rate was observed, followed by a decrease in the percentage of embryos reaching the blastocyst stage. When FBS was replaced by a defined protein supplementation, the best results were obtained with Synthetic Serum Substitute.
CONCLUSIONS: Adequate protein supplementation of the IVM medium optimizes the fertilization rate and the development of bovine IVM oocytes. The implication of these results in the human field is discussed.
Mots-clé
Animals, Cattle, Cercopithecus aethiops, Coculture Techniques, Culture Media, Female, Fertilization in Vitro/methods, Fertilization in Vitro/veterinary, Fetal Blood/physiology, Humans, Male, Oocytes/growth & development, Oocytes/physiology, Ovarian Follicle/growth & development, Ovarian Follicle/physiology, Proteins/pharmacology, Serum Albumin, Bovine/physiology, Vero Cells
Pubmed
Web of science
Création de la notice
25/01/2008 16:39
Dernière modification de la notice
20/08/2019 15:14
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