Bioluminescent orthotopic mouse models of human localized non-small cell lung cancer: feasibility and identification of circulating tumour cells.

Détails

ID Serval
serval:BIB_5B9DC51765EA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Bioluminescent orthotopic mouse models of human localized non-small cell lung cancer: feasibility and identification of circulating tumour cells.
Périodique
PloS one
Auteur⸱e⸱s
Mordant P., Loriot Y., Lahon B., Castier Y., Lesèche G., Soria J.C., Vozenin M.C., Decraene C., Deutsch E.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
6
Numéro
10
Pages
e26073
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Preclinical models of non-small cell lung cancer (NSCLC) require better clinical relevance to study disease mechanisms and innovative therapeutics. We sought to compare and refine bioluminescent orthotopic mouse models of human localized NSCLC.
Athymic nude mice underwent subcutaneous injection (group 1-SC, n = 15, control), percutaneous orthotopic injection (group 2-POI, n = 30), surgical orthotopic implantation of subcutaneously grown tumours (group 3-SOI, n = 25), or transpleural orthotopic injection (group 4-TOI, n = 30) of A549-luciferase cells. Bioluminescent in vivo imaging was then performed weekly. Circulating tumour cells (CTCs) were searched using Cellsearch® system in SC and TOI models.
Group 2-POI was associated with unexpected direct pleural spreading of the cellular solution in 53% of the cases, forbidding further evaluation of any localized lung tumour. Group 3-SOI was characterized by high perioperative mortality, initially localized lung tumours, and local evolution. Group 4-TOI was associated with low perioperative mortality, initially localized lung tumours, loco regional extension, and distant metastasis. CTCs were detected in 83% of nude mice bearing subcutaneous or orthotopic NSCLC tumours.
Transpleural orthotopic injection of A549-luc cells in nude mouse lung induces localized tumour, followed by lymphatic extension and specific mortality, and allowed the first time identification of CTCs in a NSCLC mice model.

Mots-clé
Animals, Carcinoma, Non-Small-Cell Lung/pathology, Cell Line, Tumor, Disease Models, Animal, Feasibility Studies, Female, Follow-Up Studies, Humans, Luminescent Measurements/methods, Lung Neoplasms/pathology, Mice, Neoplasm Transplantation, Neoplastic Cells, Circulating/pathology, Survival Analysis
Pubmed
Web of science
Open Access
Oui
Création de la notice
30/04/2018 15:15
Dernière modification de la notice
20/08/2019 14:14
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