Determining Macrophage Polarization upon Metabolic Perturbation.

Détails

ID Serval
serval:BIB_5B2518331659
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Determining Macrophage Polarization upon Metabolic Perturbation.
Périodique
Methods in molecular biology
Auteur(s)
Liu P.S., Ho P.C.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2019
Peer-reviewed
Oui
Volume
1862
Pages
173-186
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Metabolic reprograming controlling macrophage activation and function is emerging as new regulatory circuit on shaping immune responses. Generally, lipopolysaccharides (LPS)-induced pro-inflammatory activated macrophages, known as M1 macrophages, display higher glycolysis. In contrast, interleukin-4 (IL-4)-skewed anti-inflammatory activated macrophages, known as M2 macrophages, mainly rely on oxidative phosphorylation for their bioenergetic demands. Emerging evidence reveals that these metabolic preferences further fine-tune macrophage polarization process, including signaling cascades and epigenetic reprogramming. Thus, specific nutrient microenvironments may affect inflammatory responses of macrophages by intervening these metabolic machineries. How to measure the metabolic switch of macrophages both in vitro and in vivo is an important issue for understanding immunometabolic regulations in macrophages. Here, we describe a basic protocol for examining how glutamine metabolism affects macrophage polarization by using the Extracellular Flux (XF <sup>(e)</sup> 96) Analyzer (Seahorse Bioscience), which takes real-time measurements of oxidative phosphorylation and glycolysis. We also present a detailed procedure for detecting the expression of inflammatory genes in polarized macrophages under glutamine-replete or -deprived conditions.
Mots-clé
Animals, Biomarkers/analysis, Biomarkers/metabolism, Cells, Cultured, Glutamine/metabolism, Glycolysis/immunology, Lipopolysaccharides/immunology, Macrophage Activation/immunology, Macrophages/immunology, Macrophages/metabolism, Mass Spectrometry/instrumentation, Mass Spectrometry/methods, Metabolic Flux Analysis/instrumentation, Metabolic Flux Analysis/methods, Metabolic Networks and Pathways/immunology, Metabolomics/instrumentation, Metabolomics/methods, Mice, Mice, Inbred C57BL, Oxidative Phosphorylation, Oxygen Consumption/immunology, Primary Cell Culture/instrumentation, Primary Cell Culture/methods, Specific Pathogen-Free Organisms, Extracellular acidification, Extracellular flux analyzer, Glutamine metabolism, Macrophage polarization, Oxygen consumption
Pubmed
Création de la notice
05/11/2018 9:17
Dernière modification de la notice
20/08/2019 15:14
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