A microfabricated nerve-on-a-chip platform for rapid assessment of neural conduction in explanted peripheral nerve fibers.
Détails
ID Serval
serval:BIB_5AE452437453
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A microfabricated nerve-on-a-chip platform for rapid assessment of neural conduction in explanted peripheral nerve fibers.
Périodique
Nature communications
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Statut éditorial
Publié
Date de publication
23/10/2018
Peer-reviewed
Oui
Volume
9
Numéro
1
Pages
4403
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Résumé
Peripheral nerves are anisotropic and heterogeneous neural tissues. Their complex physiology restricts realistic in vitro models, and high resolution and selective probing of axonal activity. Here, we present a nerve-on-a-chip platform that enables rapid extracellular recording and axonal tracking of action potentials collected from tens of myelinated fibers. The platform consists of microfabricated stimulation and recording microchannel electrode arrays. First, we identify conduction velocities of action potentials traveling through the microchannel and propose a robust data-sorting algorithm using velocity selective recording. We optimize channel geometry and electrode spacing to enhance the algorithm reliability. Second, we demonstrate selective heat-induced neuro-inhibition of peripheral nerve activity upon local illumination of a conjugated polymer (P3HT) blended with a fullerene derivative (PCBM) coated on the floor of the microchannel. We demonstrate the nerve-on-a-chip platform is a versatile tool to optimize the design of implantable peripheral nerve interfaces and test selective neuromodulation techniques ex vivo.
Mots-clé
Algorithms, Animals, Hot Temperature, Lab-On-A-Chip Devices, Male, Microtechnology/methods, Neural Conduction/physiology, Neural Inhibition, Peripheral Nerves/physiology, Polymers/chemistry, Rats, Inbred Lew
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/03/2024 11:50
Dernière modification de la notice
22/03/2024 8:25