Lung epithelial Na channel subunits are differentially regulated during development and by steroids

Détails

ID Serval
serval:BIB_5A9DDE4B8924
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Lung epithelial Na channel subunits are differentially regulated during development and by steroids
Périodique
American Journal of Physiology
Auteur(s)
Tchepichev  S., Ueda  J., Canessa  C., Rossier  B. C., O'Brodovich  H.
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
09/1995
Volume
269
Numéro
3 Pt 1
Pages
C805-12
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
Because the alpha-subunit of the rat lung epithelial Na channel (rENaC) is not expressed until late fetal gestation, the developmental immaturity of alpha-rENaC may be involved in the premature fetal lung's inability to mount a Na-absorptive response to appropriate agonists. As previous work has shown that the beta- and gamma-rENaC subunits of the Na channel are required for maximal alpha-rENaC activity, we determined their developmental expression in the fetal lung. In addition, because thyroid and corticosteroid therapy can mature the in vivo fetal lamb lung's ability to transport Na, we wished to determine whether such treatment increased the expression of alpha-, beta-, and gamma-rENaC. Lungs were harvested from normal rat fetuses of 17 through 22 days gestation (term = 22 days), normal rat pups during the first week of life, and adult rats. Initial expression of alpha-rENaC was detected at 19 days gestation and progressively increased in utero. beta- and gamma-rENaC mRNA were not detected until 21 and 22 days gestation, and then only at very low levels. During the first week after birth, the levels of alpha-rENaC declined, whereas beta- and gamma-rENaC mRNA levels increased. This pre- and postnatal pattern of alpha-rENaC expression correlates with the endogenous glucocorticosteroid levels in the fetus and the rat pup's early postnatal corticosteroid resistance. Combined or separate treatment of pregnant rats (16 through 22 days gestational age) with thyroid-releasing hormone (TRH) and/or dexamethasone (Dex) for 48 h showed that Dex, but not TRH, could increase fetal lung alpha-rENaC mRNA levels.(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Adrenal Cortex Hormones/*pharmacology Aging/*metabolism Animals Animals, Newborn Dexamethasone/pharmacology Embryonic and Fetal Development Epithelium/embryology/metabolism Fetus/*metabolism/physiology Lung/*embryology/*metabolism Rats Sodium Channels/drug effects/*metabolism Sodium-Potassium-Exchanging ATPase/metabolism Thyrotropin-Releasing Hormone/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 14:00
Dernière modification de la notice
20/08/2019 15:13
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